PMID- 16949621
OWN - NLM
STAT- MEDLINE
DCOM- 20070112
LR  - 20211203
IS  - 0028-3908 (Print)
IS  - 0028-3908 (Linking)
VI  - 51
IP  - 5
DP  - 2006 Oct
TI  - Binge administration of 3,4-methylenedioxymethamphetamine ("ecstasy") impairs the 
      survival of neural precursors in adult rat dentate gyrus.
PG  - 967-73
AB  - 3,4-Methylenedioxymethamphetamine (MDMA) is a potent stimulant and hallucinogenic 
      drug whose ability to regulate neurogenesis in the adult has not been previously 
      investigated. We used 5'-bromo-2-deoxyuridine (BrdU) and Ki-67 as mitotic 
      markers, and doublecortin (DCX) as a marker of immature neurons, to study 
      proliferation, survival and maturation of adult-generated cells in the dentate 
      gyrus (DG) of the hippocampus following binge administration of MDMA (8 
      injections of 5 mg/kg at 6 h intervals). The results showed that MDMA treatment 
      did not affect cytogenesis in the DG, but significantly decreased the survival 
      rate of cells incorporated after 2 weeks to the granular layer of the DG by ca. 
      50%, and of those remaining in the subgranular layer by ca. 30%. Two weeks after 
      exposure to MDMA the length of dendritic arbors and the number of dendritic 
      branches of immature DCX+ neurons were nearly identical to those of control rats, 
      as was the level of colocalization of BrdU with DCX. These results demonstrate 
      that binge MDMA administration does not affect the proliferation rates of 
      progenitor cells in the DG, but has deleterious effects on adult neurogenesis by 
      impairing the short-term survival of vulnerable neural precursors.
FAU - Hernandez-Rabaza, V
AU  - Hernandez-Rabaza V
AD  - Laboratory of Biopsychology and Comparative Neuroscience, Instituto Cavanilles, 
      Universidad de Valencia, Poligono de la Coma s/n, Paterna, 46980 Valencia, Spain.
FAU - Dominguez-Escriba, L
AU  - Dominguez-Escriba L
FAU - Barcia, J A
AU  - Barcia JA
FAU - Rosel, J F
AU  - Rosel JF
FAU - Romero, F J
AU  - Romero FJ
FAU - Garcia-Verdugo, J M
AU  - Garcia-Verdugo JM
FAU - Canales, J J
AU  - Canales JJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060901
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (Dcx protein, rat)
RN  - 0 (Doublecortin Domain Proteins)
RN  - 0 (Doublecortin Protein)
RN  - 0 (Hallucinogens)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Neuropeptides)
RN  - G34N38R2N1 (Bromodeoxyuridine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Behavior, Animal/drug effects
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Differentiation/drug effects/physiology
MH  - Cell Survival/drug effects
MH  - Dentate Gyrus/drug effects/*pathology
MH  - Doublecortin Domain Proteins
MH  - Doublecortin Protein
MH  - Hallucinogens/*administration & dosage
MH  - Immunohistochemistry/methods
MH  - Ki-67 Antigen/metabolism
MH  - Male
MH  - Microtubule-Associated Proteins/metabolism
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*administration & dosage
MH  - Neurons/*drug effects
MH  - Neuropeptides/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Stem Cells/*drug effects
EDAT- 2006/09/05 09:00
MHDA- 2007/01/16 09:00
CRDT- 2006/09/05 09:00
PHST- 2006/04/26 00:00 [received]
PHST- 2006/06/06 00:00 [revised]
PHST- 2006/06/12 00:00 [accepted]
PHST- 2006/09/05 09:00 [pubmed]
PHST- 2007/01/16 09:00 [medline]
PHST- 2006/09/05 09:00 [entrez]
AID - S0028-3908(06)00190-0 [pii]
AID - 10.1016/j.neuropharm.2006.06.019 [doi]
PST - ppublish
SO  - Neuropharmacology. 2006 Oct;51(5):967-73. doi: 10.1016/j.neuropharm.2006.06.019. 
      Epub 2006 Sep 1.