PMID- 1695010
OWN - NLM
STAT- MEDLINE
DCOM- 19900815
LR  - 20220318
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 87
IP  - 13
DP  - 1990 Jul
TI  - Minimally modified low density lipoprotein induces monocyte chemotactic protein 1 
      in human endothelial cells and smooth muscle cells.
PG  - 5134-8
AB  - After exposure to low density lipoprotein (LDL) that had been minimally modified 
      by oxidation (MM-LDL), human endothelial cells (EC) and smooth muscle cells (SMC) 
      cultured separately or together produced 2- to 3-fold more monocyte chemotactic 
      activity than did control cells or cells exposed to freshly isolated LDL. This 
      increase in monocyte chemotactic activity was paralleled by increases in mRNA 
      levels for a monocyte chemotactic protein 1 (MCP-1) that is constitutively 
      produced by the human glioma U-105MG cell line. Antibody that had been prepared 
      against cultured baboon smooth muscle cell chemotactic factor (anti-SMCF) did not 
      inhibit monocyte migration induced by the potent bacterial chemotactic factor 
      f-Met-Leu-Phe. However, anti-SMCF completely inhibited the monocyte chemotactic 
      activity found in the media of U-105MG cells, EC, and SMC before and after 
      exposure to MM-LDL. Moreover, monocyte migration into the subendothelial space of 
      a coculture of EC and SMC that had been exposed to MM-LDL was completely 
      inhibited by anti-SMCF. Anti-SMCF specifically immunoprecipitated 10-kDa and 
      12.5-kDa proteins from EC. Incorporation of [35S]methionine into the 
      immunoprecipitated proteins paralleled the monocyte chemotactic activity found in 
      the medium of MM-LDL stimulated EC and the levels of MCP-1 mRNA found in the EC. 
      We conclude that (i) SMCF is in fact MCP-1 and (ii) MCP-1 is induced by MM-LDL.
FAU - Cushing, S D
AU  - Cushing SD
AD  - Department of Medicine, UCLA School of Medicine 90024-1679.
FAU - Berliner, J A
AU  - Berliner JA
FAU - Valente, A J
AU  - Valente AJ
FAU - Territo, M C
AU  - Territo MC
FAU - Navab, M
AU  - Navab M
FAU - Parhami, F
AU  - Parhami F
FAU - Gerrity, R
AU  - Gerrity R
FAU - Schwartz, C J
AU  - Schwartz CJ
FAU - Fogelman, A M
AU  - Fogelman AM
LA  - eng
GR  - HL26890/HL/NHLBI NIH HHS/United States
GR  - HL30568/HL/NHLBI NIH HHS/United States
GR  - HL38390/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sulfur Radioisotopes)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Aorta/drug effects/*metabolism
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*biosynthesis/genetics/pharmacology
MH  - Chemotaxis, Leukocyte/drug effects
MH  - DNA/genetics
MH  - Endothelium, Vascular/drug effects/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Lipoproteins, LDL/*pharmacology
MH  - Methionine/metabolism
MH  - Molecular Sequence Data
MH  - Monocytes/drug effects/physiology
MH  - Muscle, Smooth, Vascular/drug effects/*metabolism
MH  - Oligonucleotide Probes
MH  - Oxidation-Reduction
MH  - RNA/genetics/isolation & purification
MH  - RNA, Messenger/genetics
MH  - Sulfur Radioisotopes
MH  - Transcription, Genetic/drug effects
PMC - PMC54276
EDAT- 1990/07/01 00:00
MHDA- 1990/07/01 00:01
PMCR- 1991/01/01
CRDT- 1990/07/01 00:00
PHST- 1990/07/01 00:00 [pubmed]
PHST- 1990/07/01 00:01 [medline]
PHST- 1990/07/01 00:00 [entrez]
PHST- 1991/01/01 00:00 [pmc-release]
AID - 10.1073/pnas.87.13.5134 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1990 Jul;87(13):5134-8. doi: 10.1073/pnas.87.13.5134.