PMID- 16951573
OWN - NLM
STAT- MEDLINE
DCOM- 20061018
LR  - 20071115
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 17
IP  - 12
DP  - 2006 Aug 21
TI  - Differential brain-derived neurotrophic factor and transforming growth 
      factor-beta expression in the rat cochlea following deafness.
PG  - 1297-301
AB  - Brain derived neurotrophic factor (BDNF) and transforming growth factor-beta 
      (TGFbeta) subtypes have demonstrated their importance in cochlear functions. The 
      aim of this study was to determine gene and protein expression patterns of BDNF, 
      TGFbeta1/2 and their main receptors trkB and TGFbetaR1/R2 in the auditory nerve 
      and inferior colliculus of normal hearing and deafened rats by 
      reverse-transcriptase polymerase chain reaction and immunohistochemistry. 
      Deafening was performed by cochlear injection of neomycin (10%). Significant gene 
      expression changes were not found in the inferior colliculus after deafness; 
      however, in the auditory nerve, BDNF, TGFbeta1 and TGFbetaR1 mRNA in particular 
      were upregulated. Additionally, BDNF protein and the cytokines were found to be 
      expressed in the auditory nerve after hair cell loss. These data indicate the 
      importance of BDNF and TGFbeta1 as endogenous survival factors.
FAU - Wissel, Kirsten
AU  - Wissel K
AD  - Department of Otolaryngology, Medical University of Hannover, Hannover, Germany.
FAU - Wefstaedt, Patrick
AU  - Wefstaedt P
FAU - Miller, Josef M
AU  - Miller JM
FAU - Lenarz, Thomas
AU  - Lenarz T
FAU - Stover, Timo
AU  - Stover T
LA  - eng
GR  - DC03820/DC/NIDCD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Blotting, Northern/methods
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cochlea/*metabolism
MH  - Deafness/metabolism/*pathology
MH  - Disease Models, Animal
MH  - Gene Expression Regulation/*physiology
MH  - Immunohistochemistry/methods
MH  - Inferior Colliculi/metabolism
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Receptors, Transforming Growth Factor beta/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Transforming Growth Factor beta/genetics/*metabolism
MH  - Vestibulocochlear Nerve/metabolism
EDAT- 2006/09/05 09:00
MHDA- 2006/10/19 09:00
CRDT- 2006/09/05 09:00
PHST- 2006/09/05 09:00 [pubmed]
PHST- 2006/10/19 09:00 [medline]
PHST- 2006/09/05 09:00 [entrez]
AID - 00001756-200608210-00014 [pii]
AID - 10.1097/01.wnr.0000233088.92839.23 [doi]
PST - ppublish
SO  - Neuroreport. 2006 Aug 21;17(12):1297-301. doi: 
      10.1097/01.wnr.0000233088.92839.23.