PMID- 16951938
OWN - NLM
STAT- MEDLINE
DCOM- 20071024
LR  - 20220321
IS  - 0340-5761 (Print)
IS  - 0340-5761 (Linking)
VI  - 81
IP  - 1
DP  - 2007 Jan
TI  - Di-(2-ethylhexyl)-phthalate affects lipid profiling in fetal rat brain upon 
      maternal exposure.
PG  - 57-62
AB  - Lipids, especially essential fatty acids (EFAs), play critical roles in guiding 
      proper fetal development. Exposure to xenobiotics that may alter the fetal supply 
      of EFAs/lipids could potentially lead to fetotoxicity. In this study, we 
      investigated the effects of the peroxisome proliferator chemical, 
      di-(2-ethylhexyl)-phthalate (DEHP), on the lipid metabolomic profile of the rat 
      fetal brain upon maternal exposure during gestation. Female Sprague-Dawley rats 
      were orally gavaged with a control vehicle or DEHP (1,500 mg/kg) from gestational 
      day (GD) 0 to GD 19 and fetal brain tissue was isolated at GD 20. The 
      concentrations of 11 lipid classes [free fatty acid, free cholesterol (FC), 
      cholesterol ester (CE), diacylglycerol (DAG), triacylglyceride, 
      phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine (PS), 
      lysophosphatidylcholine (LYPC), cardiolipin, and sphingomyelin (SM)] were 
      determined, as well as the differences in the composition of individual fatty 
      acids. The total lipid concentration decreased with DEHP exposure, particularly 
      for FC and SM, by 33 and 54%, respectively. The same trend was observed in the 
      fatty acid compositions, particularly the unsaturated fatty acids, where a 
      greater decrease was observed with longer fatty acid chain length. The 
      compositions of docosahexaenoic acid decreased significantly in five lipid 
      classes (P < 0.05), including CE (43%), DAG (60%), PS (33%), LYPC (35%), and SM 
      (40%). In contrast, the most remarkable reduction of arachidonic acid presented 
      in two lipid classes, CE and LYPC, with a decrease of up to 33%. These results 
      suggest that in utero exposure to DEHP alters the lipid metabolome in the fetal 
      brain, which may lead to aberrant neurodevelopment.
FAU - Xu, Yan
AU  - Xu Y
AD  - Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State 
      University of New Jersey, 160 Frelinghuysen Road, Piscataway, NJ 08854, USA.
FAU - Agrawal, Shruti
AU  - Agrawal S
FAU - Cook, Thomas J
AU  - Cook TJ
FAU - Knipp, Gregory T
AU  - Knipp GT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060902
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (Cholesterol Esters)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fatty Acids, Monounsaturated)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Lipids)
RN  - 0 (Lysophosphatidylcholines)
RN  - 0 (Triglycerides)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - C42K0PH13C (Diethylhexyl Phthalate)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Arachidonic Acid/analysis
MH  - Brain/*drug effects/embryology/metabolism
MH  - Brain Chemistry/drug effects
MH  - Cholesterol Esters/analysis
MH  - Diethylhexyl Phthalate/*toxicity
MH  - Docosahexaenoic Acids/analysis
MH  - Environmental Pollutants/chemistry/toxicity
MH  - Fatty Acids, Monounsaturated/analysis
MH  - Fatty Acids, Omega-3/analysis
MH  - Fatty Acids, Unsaturated/analysis
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry/methods
MH  - Lipid Metabolism/drug effects
MH  - Lipids/*analysis/chemistry
MH  - Lysophosphatidylcholines/analysis
MH  - Male
MH  - Maternal Exposure
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Triglycerides/analysis
EDAT- 2006/09/05 09:00
MHDA- 2007/10/25 09:00
CRDT- 2006/09/05 09:00
PHST- 2006/06/06 00:00 [received]
PHST- 2006/07/31 00:00 [accepted]
PHST- 2006/09/05 09:00 [pubmed]
PHST- 2007/10/25 09:00 [medline]
PHST- 2006/09/05 09:00 [entrez]
AID - 10.1007/s00204-006-0143-8 [doi]
PST - ppublish
SO  - Arch Toxicol. 2007 Jan;81(1):57-62. doi: 10.1007/s00204-006-0143-8. Epub 2006 Sep 
      2.