PMID- 16952194
OWN - NLM
STAT- MEDLINE
DCOM- 20070227
LR  - 20191210
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 26
IP  - 11
DP  - 2006 Nov
TI  - Validation of DNA probes for preimplantation genetic diagnosis (PGD) by 
      fluorescence in situ hybridization (FISH) R1.
PG  - 1042-50
AB  - BACKGROUND: Preimplantation genetic diagnosis (PGD) by fluorescence in situ 
      hybridization (FISH) is being employed increasingly by medical centers and 
      private companies. Validation of any clinical assay, particularly one with novel 
      applications such as PGD by FISH, is of critical importance in the clinical 
      setting. This importance is recognized by both the College of American 
      Pathologists (CAP) and the American College of Medical Genetics (ACMG), who 
      recommend validation of FISH assays in the clinical setting. Validation of FISH 
      assays for PGD is especially significant, as only one or two cells (blastomeres) 
      will be available for testing of a given embryo. METHODS: We have developed 
      validation protocols for a variety of FISH assays, including sex identification, 
      structural chromosomal aneusomy, and aneuploidy screening with the Vysis, Inc., 
      PGT probe panel. RESULTS: Our validation results show good individual performance 
      of commercially available probes, and decreasing overall efficiency as the number 
      of probes included in an assay increases.
FAU - Weremowicz, Stanislawa
AU  - Weremowicz S
AD  - Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA.
FAU - Sandstrom, Deborah J
AU  - Sandstrom DJ
FAU - Morton, Cynthia C
AU  - Morton CC
FAU - Miron, Patricia M
AU  - Miron PM
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (DNA Probes)
SB  - IM
MH  - *DNA Probes
MH  - Female
MH  - Genetic Diseases, Inborn/*diagnosis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pregnancy
MH  - Preimplantation Diagnosis/*methods
EDAT- 2006/09/05 09:00
MHDA- 2007/02/28 09:00
CRDT- 2006/09/05 09:00
PHST- 2006/09/05 09:00 [pubmed]
PHST- 2007/02/28 09:00 [medline]
PHST- 2006/09/05 09:00 [entrez]
AID - 10.1002/pd.1552 [doi]
PST - ppublish
SO  - Prenat Diagn. 2006 Nov;26(11):1042-50. doi: 10.1002/pd.1552.