PMID- 16952295
OWN - NLM
STAT- MEDLINE
DCOM- 20061121
LR  - 20230124
IS  - 1600-6135 (Print)
IS  - 1600-6135 (Linking)
VI  - 6
IP  - 11
DP  - 2006 Nov
TI  - Maturation-resistant dendritic cells induce hyporesponsiveness in alloreactive 
      CD45RA+ and CD45RO+ T-cell populations.
PG  - 2580-91
AB  - Dendritic cells (DCs) play a crucial role in the induction of antigen-specific 
      immunity and tolerance. Considering in vivo application of DCs prior to human 
      organ transplantation, a protocol to develop tolerogenic DCs that not only induce 
      unresponsiveness in naive (CD45RA+) T cells, but also in alloreactive memory 
      (CD45RO+) T cells is required. The present study shows that dexamethasone (Dex) 
      alters the differentiation of human monocyte-derived DCs. DexDCs cocultured with 
      allogeneic CD4+ T cells induced low proliferating and low IFNgamma producing T 
      cells. This is caused by lack of both costimulation via CD28 and hampered 
      production of a soluble factor, as well as additional active suppression via 
      B7-H1 and IL-10. T cells primed by DexDCs demonstrated hyporesponsiveness upon 
      restimulation with mature DCs seemingly via the induction of anergy, since these 
      cells showed no enhanced apoptosis and only a limited suppressive capacity. 
      Interestingly, not only cocultures of allogeneic CD45RA+, but also of CD45RO+ T 
      cells with DexDCs rendered T-cell populations hyporesponsive to restimulation 
      with mature DCs. The finding that also alloreactive memory T cells can be 
      regulated supports the rationale of cell-based therapies to obtain 
      allograft-specific tolerance in transplant recipients.
FAU - Woltman, A M
AU  - Woltman AM
AD  - Department of Nephrology, Leiden University Medical Center, Leiden, The 
      Netherlands.
FAU - van der Kooij, S W
AU  - van der Kooij SW
FAU - de Fijter, J W
AU  - de Fijter JW
FAU - van Kooten, C
AU  - van Kooten C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060904
PL  - United States
TA  - Am J Transplant
JT  - American journal of transplantation : official journal of the American Society of 
      Transplantation and the American Society of Transplant Surgeons
JID - 100968638
RN  - 0 (Antigens, CD)
RN  - 0 (B7-H1 Antigen)
RN  - 0 (CD274 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (Isoantigens)
RN  - 130068-27-8 (Interleukin-10)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Antigens, CD/immunology
MH  - B7-H1 Antigen
MH  - Cytokines/analysis/metabolism
MH  - Dendritic Cells/*immunology
MH  - Dexamethasone/pharmacology
MH  - Flow Cytometry
MH  - Humans
MH  - Interleukin-10/immunology
MH  - Isoantigens/immunology
MH  - Leukocyte Common Antigens/*immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Monocytes/drug effects/immunology
MH  - T-Lymphocytes/*immunology
EDAT- 2006/09/06 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/09/06 09:00
PHST- 2006/09/06 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/09/06 09:00 [entrez]
AID - S1600-6135(22)02867-2 [pii]
AID - 10.1111/j.1600-6143.2006.01520.x [doi]
PST - ppublish
SO  - Am J Transplant. 2006 Nov;6(11):2580-91. doi: 10.1111/j.1600-6143.2006.01520.x. 
      Epub 2006 Sep 4.