PMID- 16954384
OWN - NLM
STAT- MEDLINE
DCOM- 20061113
LR  - 20211203
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 26
IP  - 20
DP  - 2006 Oct
TI  - Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in 
      embryonic stem cells.
PG  - 7772-82
AB  - Although the POU transcription factor Oct3/4 is pivotal in maintaining self 
      renewal of embryonic stem (ES) cells, little is known of its molecular 
      mechanisms. We previously reported that the N-terminal transactivation domain of 
      Oct3/4 is required for activation of Lefty1 expression (H. Niwa, S. Masui, I. 
      Chambers, A. G. Smith, and J. Miyazaki, Mol. Cell. Biol. 22:1526-1536, 2002). 
      Here we test whether Lefty1 is a direct target of Oct3/4. We identified an ES 
      cell-specific enhancer upstream of the Lefty1 promoter that contains binding 
      sites for Oct3/4 and Sox2. Unlike other known Oct3/4-Sox2-dependent enhancers, 
      however, this enhancer element could not be activated by Oct3/4 and Sox2 in 
      differentiated cells. By functional screening of ES-specific transcription 
      factors, we found that Kruppel-like factor 4 (Klf4) cooperates with Oct3/4 and 
      Sox2 to activate Lefty1 expression, and that Klf4 acts as a mediating factor that 
      specifically binds to the proximal element of the Lefty1 promoter. DNA microarray 
      analysis revealed that a subset of putative Oct3/4 target genes may be regulated 
      in the same manner. Our findings shed light on a novel function of Oct3/4 in ES 
      cells.
FAU - Nakatake, Yuhki
AU  - Nakatake Y
AD  - Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, 
      Minatojima-Minamimachi 2-2-3, Chu-o-ku, Kobe 650-0047, Japan.
FAU - Fukui, Nobutaka
AU  - Fukui N
FAU - Iwamatsu, Yuko
AU  - Iwamatsu Y
FAU - Masui, Shinji
AU  - Masui S
FAU - Takahashi, Kadue
AU  - Takahashi K
FAU - Yagi, Rika
AU  - Yagi R
FAU - Yagi, Kiyohito
AU  - Yagi K
FAU - Miyazaki, Jun-Ichi
AU  - Miyazaki J
FAU - Matoba, Ryo
AU  - Matoba R
FAU - Ko, Minoru S H
AU  - Ko MS
FAU - Niwa, Hitoshi
AU  - Niwa H
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060905
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (HMGB Proteins)
RN  - 0 (KLF4 protein, human)
RN  - 0 (Klf4 protein, mouse)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Left-Right Determination Factors)
RN  - 0 (Lefty1 protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Organic Cation Transport Proteins)
RN  - 0 (Sex-Determining Region Y Protein)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Cell Differentiation
MH  - Conserved Sequence
MH  - Embryo, Mammalian/cytology/metabolism
MH  - Gene Expression/genetics
MH  - Genes, Reporter/genetics
MH  - HMGB Proteins/genetics/*metabolism
MH  - Humans
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors/genetics/*metabolism
MH  - Left-Right Determination Factors
MH  - Membrane Proteins/*genetics/metabolism
MH  - Mice
MH  - Organic Cation Transport Proteins/genetics/*metabolism
MH  - Promoter Regions, Genetic/*genetics
MH  - Protein Binding
MH  - Sex-Determining Region Y Protein/genetics/*metabolism
MH  - Stem Cells/cytology/*metabolism
MH  - Transforming Growth Factor beta/*genetics/metabolism
PMC - PMC1636862
EDAT- 2006/09/07 09:00
MHDA- 2006/11/14 09:00
PMCR- 2007/02/01
CRDT- 2006/09/07 09:00
PHST- 2006/09/07 09:00 [pubmed]
PHST- 2006/11/14 09:00 [medline]
PHST- 2006/09/07 09:00 [entrez]
PHST- 2007/02/01 00:00 [pmc-release]
AID - MCB.00468-06 [pii]
AID - 0468-06 [pii]
AID - 10.1128/MCB.00468-06 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2006 Oct;26(20):7772-82. doi: 10.1128/MCB.00468-06. Epub 2006 Sep 
      5.