PMID- 16954668
OWN - NLM
STAT- MEDLINE
DCOM- 20060918
LR  - 20071114
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 114
IP  - 3-4
DP  - 2006
TI  - Molecular cytogenetic characterization of chromosome 9-derived material in a 
      human thyroid cancer cell line.
PG  - 284-91
AB  - The incidence of papillary thyroid carcinoma (PTC) increases significantly after 
      exposure of the head and neck region to ionizing radiation, yet we know neither 
      the steps involved in malignant transformation of thyroid epithelium nor the 
      specific carcinogenic mode of action of radiation. Such increased tumor frequency 
      became most evident in children after the 1986 nuclear accident in Chernobyl, 
      Ukraine. In the eight years following the accident, the average incidence of 
      childhood PTCs (chPTC) increased 70-fold in Belarus, 200-fold in Gomel, 10-fold 
      in the Ukraine and 50-fold in Tschnigov, Kiev, Rovno, Shitomyr and Tscherkassy 
      compared to the rate of about 1 tumor incidence per 106 children per year prior 
      to 1986 (Likhtarev et al., 1995; Sobolev et al., 1997; Jacob et al., 1998). To 
      study the etiology of radiation-induced thyroid cancer, we formed an 
      international consortium to investigate chromosomal changes and altered gene 
      expression in cases of post-Chernobyl chPTC. Our approach is based on karyotyping 
      of primary cultures established from chPTC specimens, establishment of cell lines 
      and studies of genotype-phenotype relationships through high resolution 
      chromosome analysis, DNA/cDNA micro-array studies, and mouse xenografts that test 
      for tumorigenicity. Here, we report the application of fluorescence in situ 
      hybridization (FISH)-based techniques for the molecular cytogenetic 
      characterization of a highly tumorigenic chPTC cell line, S48TK, and its 
      subclones. Using chromosome 9 rearrangements as an example, we describe a new 
      approach termed 'BAC-FISH' to rapidly delineate chromosomal breakpoints, an 
      important step towards a better understanding of the formation of translocations 
      and their functional consequences.
CI  - Copyright 2006 S. Karger AG, Basel.
FAU - Weier, H-U G
AU  - Weier HU
AD  - Life Sciences Division, University of California, E.O. Lawrence Berkeley National 
      Laboratory, Berkeley, CA 94720, USA. ulliweier@hotmail.com
FAU - Tuton, T B
AU  - Tuton TB
FAU - Ito, Y
AU  - Ito Y
FAU - Chu, L W
AU  - Chu LW
FAU - Lu, C-M
AU  - Lu CM
FAU - Baumgartner, A
AU  - Baumgartner A
FAU - Zitzelsberger, H F
AU  - Zitzelsberger HF
FAU - Weier, J F
AU  - Weier JF
LA  - eng
GR  - CA88258/CA/NCI NIH HHS/United States
GR  - HD44313/HD/NICHD NIH HHS/United States
GR  - HD45736/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA Probes)
SB  - IM
MH  - Cell Line, Tumor
MH  - Chromosome Painting/methods
MH  - *Chromosomes, Human, Pair 9
MH  - Cytogenetic Analysis
MH  - DNA Probes
MH  - Humans
MH  - Karyotyping
MH  - Metaphase
MH  - Nucleic Acid Hybridization
MH  - Thyroid Neoplasms/*genetics/pathology
EDAT- 2006/09/07 09:00
MHDA- 2006/09/19 09:00
CRDT- 2006/09/07 09:00
PHST- 2006/01/04 00:00 [received]
PHST- 2006/02/03 00:00 [accepted]
PHST- 2006/09/07 09:00 [pubmed]
PHST- 2006/09/19 09:00 [medline]
PHST- 2006/09/07 09:00 [entrez]
AID - 94215 [pii]
AID - 10.1159/000094215 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2006;114(3-4):284-91. doi: 10.1159/000094215.