PMID- 16954669
OWN - NLM
STAT- MEDLINE
DCOM- 20060918
LR  - 20071115
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 114
IP  - 3-4
DP  - 2006
TI  - Cytogenetic characteristics of a murine in vitro model for the human anaplastic 
      large cell lymphoma (ALCL).
PG  - 292-5
AB  - Anaplastic large cell lymphoma (ALCL) is an entity of non-Hodgkin lymphomas (NHL) 
      that often occurs in young children and adolescents. In the majority of cases, 
      ALCL are of T-cell origin and contain the t(2;5)(p23;q35) leading to an NPM-ALK 
      fusion or variant ALK translocations. In addition, there is an ALK-negative 
      subtype of ALCL. The anaplastic lymphoid cell line TS1G6 established by 
      interleukin (IL)-9 transfection of T-helper cells represents a murine model of 
      this subtype. Here, we describe the cytogenetic features of this cell line using 
      spectral karyotyping (SKY) and single-color fluorescence in situ hybridization 
      (FISH). We show that TS1G6 cells exhibit a hypotetraploid karyotype with complex 
      structural alterations. Several unbalanced translocations involved the 
      chromosomal region 14E5, and different translocation partners, i.e. X?A6, 3A3 and 
      8A1. FISH analysis using a BAC clone containing c-myc confirmed the presence of 
      six copies, but also demonstrated that two loci were irregularly located, 
      indicating that additional intrachromosomal rearrangements had occurred. 
      Moreover, a duplication of the region XF2 approximately 3 was identified. 
      Furthermore, six chromosomes 15 were found, representing a trisomy 15 in a 
      tetraploid chromosome complement, indicating an altered gene dosage of the 
      oncogene c-myc located in region 15D3.
CI  - Copyright 2006 S. Karger AG, Basel.
FAU - Rudolph, C
AU  - Rudolph C
AD  - Institute of Cell and Molecular Pathology, Hannover Medical School, Hannover, 
      Germany.
FAU - Bittner, C
AU  - Bittner C
FAU - Feller, A C
AU  - Feller AC
FAU - Merz, H
AU  - Merz H
FAU - Schlegelberger, B
AU  - Schlegelberger B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Animals
MH  - *Chromosome Aberrations
MH  - Chromosome Mapping
MH  - Disease Models, Animal
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping/methods
MH  - Lymphoma, Large B-Cell, Diffuse/*genetics/*pathology
MH  - Mice
MH  - Tumor Cells, Cultured
EDAT- 2006/09/07 09:00
MHDA- 2006/09/19 09:00
CRDT- 2006/09/07 09:00
PHST- 2005/11/14 00:00 [received]
PHST- 2006/02/16 00:00 [accepted]
PHST- 2006/09/07 09:00 [pubmed]
PHST- 2006/09/19 09:00 [medline]
PHST- 2006/09/07 09:00 [entrez]
AID - 94216 [pii]
AID - 10.1159/000094216 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2006;114(3-4):292-5. doi: 10.1159/000094216.