PMID- 16955479
OWN - NLM
STAT- MEDLINE
DCOM- 20061130
LR  - 20161124
IS  - 8755-1039 (Print)
IS  - 1097-0339 (Linking)
VI  - 34
IP  - 10
DP  - 2006 Oct
TI  - Fine-needle aspiration cytology of alveolar rhabdomyosarcoma utilizing ThinPrep 
      liquid-based sample and cytospin preparations: a case confirmed by FKHR break 
      apart rearrangement by FISH probe.
PG  - 704-6
AB  - Rhabdomyosarcoma is the most common childhood sarcoma that occurs in the soft 
      tissues of the head and neck, genitourinary system, and extremities. While this 
      tumor may also be seen in young adults, it is distinctly unusual to see this 
      neoplasm beyond the fifth decade. The use of fluorescence in situ hybridization 
      (FISH) probes on fine-needle aspiration material for chromosomal analysis to 
      detect chromosomal translocations are not well established. Herein we present a 
      case of metastatic alveolar rhabdomyosarcoma originating in the nasal sinus and 
      recurring in the neck of an adult man 5 yr after his initial presentation. The 
      ThinPrep smear demonstrated isolated malignant tumor cells similar to his 
      previous histologic material. The cytologic findings of alveolar rhabdomyosarcoma 
      were confirmed utilizing a dual color break apart FISH probe which identified the 
      FKHR translocation on the cytospin preparation prepared from a ThinPrep vial and 
      processed as a cytospin preparation. In addition to routine and immunocytologic 
      staining, we demonstrate that aspirated tumor cells can be analyzed using a FISH 
      probe to identify specific chromosomal abnormalities.
FAU - Das, Kasturi
AU  - Das K
AD  - Department of Pathology, New Jersey Medical School, Newark, New Jersey 07103, 
      USA. daska@umdnj.edu
FAU - Mirani, Neena
AU  - Mirani N
FAU - Hameed, Meera
AU  - Hameed M
FAU - Pliner, Lillian
AU  - Pliner L
FAU - Aisner, Seena C
AU  - Aisner SC
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - 0 (DNA, Neoplasm)
RN  - 0 (FOXO1 protein, human)
RN  - 0 (Forkhead Box Protein O1)
RN  - 0 (Forkhead Transcription Factors)
SB  - IM
MH  - Biopsy, Fine-Needle/methods
MH  - Chromosomes, Human, Pair 13/genetics
MH  - Cytodiagnosis/methods
MH  - DNA, Neoplasm/genetics
MH  - Forkhead Box Protein O1
MH  - Forkhead Transcription Factors/*genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Gene Rearrangement/genetics
MH  - Head and Neck Neoplasms/*diagnosis/*genetics/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
MH  - Rhabdomyosarcoma, Alveolar/*diagnosis/*genetics/metabolism
MH  - Translocation, Genetic/*genetics
EDAT- 2006/09/07 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/09/07 09:00
PHST- 2006/09/07 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/09/07 09:00 [entrez]
AID - 10.1002/dc.20545 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2006 Oct;34(10):704-6. doi: 10.1002/dc.20545.