PMID- 16955639
OWN - NLM
STAT- MEDLINE
DCOM- 20061024
LR  - 20131121
IS  - 1433-6510 (Print)
IS  - 1433-6510 (Linking)
VI  - 52
IP  - 7-8
DP  - 2006
TI  - Heparin plasma sampling as an alternative to EDTA for BNP determination on the 
      Access-Beckman Coulter--effect of storage at -20 degrees C.
PG  - 393-7
AB  - To determine BNP, EDTA plasma is the only suitable specimen recommended by the 
      manufacturer. Since many laboratories, especially in Europe, use heparin plasma 
      rather than EDTA plasma for many or most of their clinical assays and in 
      particular for determination of cardiac markers (cTnI, myoglobin), it appeared 
      critical to evaluate the use of heparin plasma samples, in comparison to EDTA 
      plasma, for BNP determination on a automated immunochemiluminescent analyzer. The 
      aim of this study was first, to evaluate the use of heparin plasma samples for 
      Biosite BNP testing on the Beckman Coulter Access Immunoassay System (n=88) and 
      second, to evaluate the effect of storage at -20 degrees C, without protease 
      inhibitors, on the Biosite BNP assay. We obtained acceptable imprecision results 
      with CVs ranging from 1.7 to 11.7% regardless of the anticoagulant used. The 
      linearity of EDTA samples was good and comparable to the results observed with 
      heparin plasma. The concentration of BNP was categorized according to the 
      classification of the New York Heart Association (NYHA). With EDTA fresh samples 
      as reference anticoagulant, 90% vs. 89% of subjects were classified as 
      "concordant" with heparin fresh vs heparin frozen plasma samples, respectively. 
      After storage at -20 degrees C, only 86% of the values of EDTA frozen were 
      concordant with values of EDTA fresh. No subject varied by two NYHA classes. 
      Heparin plasma is an attractive alternative to the established EDTA samples which 
      can be used for BNP determination. This flexibility allows the simultaneous 
      determination of CK, CK-MB, cTnI and BNP on a single heparin specimen, which 
      facilitates blood collection for clinicians and nursing staff in an emergency 
      unit. In addition, our results suggest that BNP could be stored at -20 degrees C 
      for at least one month in order to perform retrospective studies.
FAU - Dupuy, Anne Marie
AU  - Dupuy AM
AD  - Department of Biochemistry, Lapeyronie Hospital, Montpellier, France.
FAU - Terrier, Nathalie
AU  - Terrier N
FAU - Dubois, Marjorie
AU  - Dubois M
FAU - Boularan, Anne Marie
AU  - Boularan AM
FAU - Cristol, Jean Paul
AU  - Cristol JP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Germany
TA  - Clin Lab
JT  - Clinical laboratory
JID - 9705611
RN  - 114471-18-0 (Natriuretic Peptide, Brain)
RN  - 9005-49-6 (Heparin)
RN  - 9G34HU7RV0 (Edetic Acid)
SB  - IM
MH  - Blood Preservation/*methods
MH  - Cryopreservation/*methods
MH  - Edetic Acid
MH  - Heparin/*blood
MH  - Immunoassay/*methods
MH  - Luminescent Measurements/methods
MH  - Natriuretic Peptide, Brain/*blood
EDAT- 2006/09/08 09:00
MHDA- 2006/10/25 09:00
CRDT- 2006/09/08 09:00
PHST- 2006/09/08 09:00 [pubmed]
PHST- 2006/10/25 09:00 [medline]
PHST- 2006/09/08 09:00 [entrez]
PST - ppublish
SO  - Clin Lab. 2006;52(7-8):393-7.