PMID- 16956324
OWN - NLM
STAT- MEDLINE
DCOM- 20070129
LR  - 20191210
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 401
IP  - 1
DP  - 2007 Jan 1
TI  - IOP1, a novel hydrogenase-like protein that modulates hypoxia-inducible 
      factor-1alpha activity.
PG  - 341-52
AB  - A central means by which mammalian cells respond to low oxygen tension is through 
      the activation of the transcription factor HIF-1 (hypoxia-inducible factor-1). 
      Under normoxic conditions, HIF-1alpha (the alpha subunit of HIF-1) is targeted 
      for rapid degradation by the ubiquitin-proteasome pathway. Under hypoxic 
      conditions, this degradation is inhibited, thereby leading to the stabilization 
      and activation of HIF-1alpha. Here, we report the identification of IOP1 
      (iron-only hydrogenase-like protein 1), a protein homologous with enzymes present 
      in anaerobic organisms that contain a distinctive iron-sulfur cluster. IOP1 is 
      present in a broad range of cell types. Knockdown of IOP1 using siRNA (small 
      interfering RNA) in mammalian cells increases protein levels of HIF-1alpha under 
      both normoxic and hypoxic conditions, and augments hypoxia-induced HRE (hypoxia 
      response element) reporter gene and endogenous HIF-1alpha target gene 
      expressions. We find that IOP1 knockdown up-regulates HIF-1alpha mRNA levels, 
      thereby providing a mechanism by which knockdown induces the observed effects. 
      The results collectively provide evidence that IOP1 is a component of the protein 
      network that regulates HIF-1alpha in mammalian cells.
FAU - Huang, Jianhe
AU  - Huang J
AD  - Department of Pathology and Laboratory Medicine, University of Pennsylvania 
      School of Medicine, Philadelphia, PA 19104, USA.
FAU - Song, Daisheng
AU  - Song D
FAU - Flores, Adrian
AU  - Flores A
FAU - Zhao, Quan
AU  - Zhao Q
FAU - Mooney, Sharon M
AU  - Mooney SM
FAU - Shaw, Leslie M
AU  - Shaw LM
FAU - Lee, Frank S
AU  - Lee FS
LA  - eng
GR  - R01 CA090261/CA/NCI NIH HHS/United States
GR  - R01 GM071459/GM/NIGMS NIH HHS/United States
GR  - R01-CA090261/CA/NCI NIH HHS/United States
GR  - R01-GM071459/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (CIAO3 protein, human)
RN  - 0 (Iron-Sulfur Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 1.12.7.2 (Hydrogenase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Blotting, Northern
MH  - Conserved Sequence
MH  - Humans
MH  - Hydrogenase/chemistry/genetics/*metabolism
MH  - Iron-Sulfur Proteins
MH  - Kidney
MH  - Molecular Sequence Data
MH  - Peptide Fragments/chemistry/metabolism
MH  - Saccharomyces cerevisiae/enzymology
MH  - Saccharomyces cerevisiae Proteins/chemistry/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
PMC - PMC1698691
EDAT- 2006/09/08 09:00
MHDA- 2007/01/30 09:00
PMCR- 2007/07/01
CRDT- 2006/09/08 09:00
PHST- 2006/09/08 09:00 [pubmed]
PHST- 2007/01/30 09:00 [medline]
PHST- 2006/09/08 09:00 [entrez]
PHST- 2007/07/01 00:00 [pmc-release]
AID - BJ20060635 [pii]
AID - bj4010341 [pii]
AID - 10.1042/BJ20060635 [doi]
PST - ppublish
SO  - Biochem J. 2007 Jan 1;401(1):341-52. doi: 10.1042/BJ20060635.