PMID- 16956600
OWN - NLM
STAT- MEDLINE
DCOM- 20070702
LR  - 20071115
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 376
IP  - 1-2
DP  - 2007 Feb
TI  - Atherogenic and inflammatory profile of human arterial endothelial cells (HUAEC) 
      in response to LDL subfractions.
PG  - 233-6
AB  - BACKGROUND: Electronegative LDL (LDL(-)) is a minor modified LDL fraction present 
      in plasma that has been demonstrated to be inflammatory in endothelial cells 
      isolated from human umbilical vein (HUVEC). METHODS: A protein array able to 
      measure 42 cytokines, chemokines and related compounds involved in atherogenesis 
      was used to determine their release into the culture medium of human arterial 
      endothelial cells (HUAEC) activated or not by two low-density lipoprotein (LDL) 
      fractions isolated from human plasma by anion-exchange chromatography. RESULTS: 
      The results of the protein array (confirmed using specific ELISAs for each 
      induced factor) revealed that HUAEC in the absence of stimuli released small 
      amounts of interleukin 8 (IL-8), monocyte chemotactic protein 1 (MCP-1) and 
      growth-related oncogene (GRO). The major native LDL fraction (named LDL(+)) 
      increased the release of these molecules and also those of interleukin 6 (IL-6) 
      and GROalpha. Compared to LDL(+), the minor modified fraction, named 
      electronegative LDL (LDL(-)), increased all these factors to a greater degree and 
      also induced the release of granulocyte-monocyte colony-stimulating factor 
      (GM-CSF) and platelet-derived growth factor B (PDGF-B). These results were 
      confirmed by ELISA. CONCLUSIONS: All these results indicate that, compared to 
      LDL(+), LDL(-) fraction promotes not only the release of proinflammatory factors 
      but also those of atherogenic factors in endothelial cells of arterial origin, 
      thereby suggesting a new role for LDL(-) in atherogenesis.
FAU - de Castellarnau, Conxita
AU  - de Castellarnau C
AD  - Department of Cell Biology, Facultat de Biologia, Universitat de Barcelona, 
      Barcelona, Spain; Advancell S.L. Barcelona Science Park, Barcelona, Spain.
FAU - Bancells, Cristina
AU  - Bancells C
FAU - Benitez, Sonia
AU  - Benitez S
FAU - Reina, Manuel
AU  - Reina M
FAU - Ordonez-Llanos, Jordi
AU  - Ordonez-Llanos J
FAU - Sanchez-Quesada, Jose Luis
AU  - Sanchez-Quesada JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060729
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (CXCL1 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Proto-Oncogene Proteins c-sis)
SB  - IM
MH  - Atherosclerosis/etiology/*metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CXCL1
MH  - Chemokines, CXC/metabolism
MH  - Culture Media, Conditioned/analysis/metabolism
MH  - Endothelium, Vascular/cytology/*drug effects/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Inflammation/chemically induced/*metabolism
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - Lipoproteins, LDL/*pharmacology
MH  - Protein Array Analysis
MH  - Proto-Oncogene Proteins c-sis/metabolism
MH  - Umbilical Arteries/cytology
EDAT- 2006/09/08 09:00
MHDA- 2007/07/03 09:00
CRDT- 2006/09/08 09:00
PHST- 2006/07/07 00:00 [received]
PHST- 2006/07/25 00:00 [revised]
PHST- 2006/07/25 00:00 [accepted]
PHST- 2006/09/08 09:00 [pubmed]
PHST- 2007/07/03 09:00 [medline]
PHST- 2006/09/08 09:00 [entrez]
AID - S0009-8981(06)00482-7 [pii]
AID - 10.1016/j.cca.2006.07.024 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2007 Feb;376(1-2):233-6. doi: 10.1016/j.cca.2006.07.024. Epub 
      2006 Jul 29.