PMID- 16956826
OWN - NLM
STAT- MEDLINE
DCOM- 20061024
LR  - 20061115
IS  - 1592-8721 (Electronic)
IS  - 0390-6078 (Linking)
VI  - 91
IP  - 9
DP  - 2006 Sep
TI  - Dual-color split signal fluorescence in situ hybridization assays for the 
      detection of CALM/AF10 in t(10;11)(p13;q14-q21)-positive acute leukemia.
PG  - 1248-51
AB  - We developed dual-color split fluorescence in situ hybridization (FISH) assays to 
      detect AF10 and/or CALM rearrangements. Among nine cases of acute leukemia with 
      translocation breakpoints at 10p13 and 11q14-21, a CALM/AF10 rearrangement was 
      found in seven and was confirmed by reverse transcriptase polymerase chain 
      reaction (RT-PCR) in all. In 2/7 cases, FISH detected CALM/AF10 in extramedullary 
      leukemic infiltrations in the mediastinum and breast. As expected, FISH was less 
      sensitive than RT-PCR for disease monitoring of CALM-AF10 positive cases. This 
      new FISH assay reliably discriminates between MLL/AF10 and CALM/AF10 genomic 
      rearrangements, identifies variant and complex CALM/AF10 translocations and 
      detects the CALM/AF10 rearrangement in extramedullary leukemic infiltrations.
FAU - La Starza, Roberta
AU  - La Starza R
AD  - Hematology and Bone Marrow Transplantation Unit, Policlinico Monteluce, via 
      Brunamonti, 06122 Perugia, Italy.
FAU - Crescenzi, Barbara
AU  - Crescenzi B
FAU - Krause, Alexandre
AU  - Krause A
FAU - Pierini, Valentina
AU  - Pierini V
FAU - Specchia, Giorgina
AU  - Specchia G
FAU - Bardi, Antonella
AU  - Bardi A
FAU - Nieddu, Rosa
AU  - Nieddu R
FAU - Ariola, Cristina
AU  - Ariola C
FAU - Nanni, Mauro
AU  - Nanni M
FAU - Diverio, Daniela
AU  - Diverio D
FAU - Aventin, Anna
AU  - Aventin A
FAU - Sborgia, Marco
AU  - Sborgia M
FAU - Martelli, Massimo F
AU  - Martelli MF
FAU - Bohlander, Stefan K
AU  - Bohlander SK
FAU - Mecucci, Cristina
AU  - Mecucci C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
RN  - 0 (AF10-CALM fusion protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia/*genetics/pathology
MH  - Leukemic Infiltration/diagnosis
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Retrospective Studies
MH  - *Translocation, Genetic
EDAT- 2006/09/08 09:00
MHDA- 2006/10/25 09:00
CRDT- 2006/09/08 09:00
PHST- 2006/09/08 09:00 [pubmed]
PHST- 2006/10/25 09:00 [medline]
PHST- 2006/09/08 09:00 [entrez]
PST - ppublish
SO  - Haematologica. 2006 Sep;91(9):1248-51.