PMID- 16957240
OWN - NLM
STAT- MEDLINE
DCOM- 20061108
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 72
IP  - 9
DP  - 2006 Sep
TI  - Comparison of Salmonella enterica serovar Typhimurium LT2 and non-LT2 salmonella 
      genomic sequences, and genotyping of salmonellae by using PCR.
PG  - 6142-51
AB  - Genes of Salmonella enterica serovar Typhimurium LT2 expected to be specifically 
      present in Salmonella were selected using the Basic Local Alignment Search Tool 
      (BLAST) program. The 152 selected genes were compared with 11 genomic sequences 
      of Salmonella serovars, including Salmonella enterica subsp. I and IIIb and 
      Salmonella bongori (V), and were clustered into 17 groups by their comparison 
      patterns. A total of 38 primer pairs were constructed to represent each of the 17 
      groups, and PCR was performed with various Salmonella subspecies including 
      Salmonella enterica subsp. I, II, IIIa, IIIb, IV, VI, and V to evaluate a 
      comprehensive DNA-based scheme for identification of Salmonella subspecies and 
      the major disease-causing Salmonella serovars. Analysis of PCR results showed 
      that Salmonella enterica subsp. I was critically divided from other subspecies, 
      and Salmonella strains belonging to S. enterica subsp. I were clustered based on 
      their serovars. In addition, genotypic relationships within S. enterica subsp. I 
      by PCR results were investigated. Also, Salmonella signature genes, Salmonella 
      enterica serovar Typhimurium signature genes, and Salmonella enterica subsp. I 
      signature genes were demonstrated based on their PCR results. The described PCR 
      method suggests a rapid and convenient method for identification of Salmonella 
      serovars that can be used by nonspecialized laboratories. Genome sequence 
      comparison can be a useful tool in epidemiologic and taxonomic studies of 
      Salmonella.
FAU - Kim, Hyun-Joong
AU  - Kim HJ
AD  - Institute of Life Sciences and Resources and Graduate School of Biotechnology, 
      Kyung Hee University, Suwon 449-701, Korea. hykim@khu.ac.kr
FAU - Park, Si-Hong
AU  - Park SH
FAU - Kim, Hae-Yeong
AU  - Kim HY
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Bacterial Typing Techniques
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - DNA, Bacterial/genetics
MH  - Genome, Bacterial
MH  - Genotype
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Salmonella enterica/classification/genetics/pathogenicity
MH  - Salmonella typhimurium/*classification/*genetics/pathogenicity
MH  - Sequence Homology, Nucleic Acid
MH  - Serotyping
MH  - Species Specificity
PMC - PMC1563604
EDAT- 2006/09/08 09:00
MHDA- 2006/11/10 09:00
PMCR- 2007/01/01
CRDT- 2006/09/08 09:00
PHST- 2006/09/08 09:00 [pubmed]
PHST- 2006/11/10 09:00 [medline]
PHST- 2006/09/08 09:00 [entrez]
PHST- 2007/01/01 00:00 [pmc-release]
AID - 72/9/6142 [pii]
AID - 0138-06 [pii]
AID - 10.1128/AEM.00138-06 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2006 Sep;72(9):6142-51. doi: 10.1128/AEM.00138-06.