PMID- 16958752
OWN - NLM
STAT- MEDLINE
DCOM- 20061019
LR  - 20061115
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 8
IP  - 10
DP  - 2006 Oct
TI  - The in situ physiology of Skermania piniformis in foams in Australian activated 
      sludge plants.
PG  - 1712-20
AB  - The in situ physiology of the filamentous bacterium Skermania piniformis 
      frequently seen in activated sludge foams in Australia was investigated. An 
      oligonucleotide probe, Spin1449, targeting the 16S rRNA of S. piniformis was 
      designed for its identification by fluorescence in situ hybridization (FISH), 
      validated with pure cultures and applied successfully to foam samples from two 
      geographically distant Australian plants. While filaments of this bacterium 
      appeared to be comparatively hydrophobic, the organism had no clear preference 
      for hydrophobic or hydrophilic substrates. In both foams examined using 
      microautoradiography (MAR), filaments selectively took up substrates under 
      aerobic and anoxic (NO(3) (-)) but not anaerobic or anoxic (NO(2) (-)) 
      conditions. Skermania piniformis assimilated oleic acid, palmitic acid, glycerol 
      and glycine. Ectoenzyme activities detected suggest that S. piniformis has an 
      ability to assimilate a greater range of substrates than might be concluded from 
      the MAR data obtained here. Based on the substrate uptake data presented here, an 
      anaerobic selector may work for controlling S. piniformis in activated sludge 
      systems.
FAU - Eales, Kathryn L
AU  - Eales KL
AD  - Biotechnology Research Centre, La Trobe University, Bendigo, Victoria 3550, 
      Australia.
FAU - Nielsen, Jeppe L
AU  - Nielsen JL
FAU - Seviour, Elizabeth M
AU  - Seviour EM
FAU - Nielsen, Per H
AU  - Nielsen PH
FAU - Seviour, Robert J
AU  - Seviour RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Actinobacteria/genetics/*physiology/ultrastructure
MH  - Autoradiography/methods
MH  - Cloning, Molecular
MH  - DNA, Bacterial/chemistry/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - New South Wales
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Queensland
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - *Water Microbiology
EDAT- 2006/09/09 09:00
MHDA- 2006/10/20 09:00
CRDT- 2006/09/09 09:00
PHST- 2006/09/09 09:00 [pubmed]
PHST- 2006/10/20 09:00 [medline]
PHST- 2006/09/09 09:00 [entrez]
AID - EMI1107 [pii]
AID - 10.1111/j.1462-2920.2006.01107.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2006 Oct;8(10):1712-20. doi: 10.1111/j.1462-2920.2006.01107.x.