PMID- 1695989
OWN - NLM
STAT- MEDLINE
DCOM- 19900904
LR  - 20190819
IS  - 0306-9877 (Print)
IS  - 0306-9877 (Linking)
VI  - 32
IP  - 2
DP  - 1990 Jun
TI  - A putative approach for gene therapy against human immunodeficiency virus (HIV).
PG  - 81-4
AB  - It is proposed here that a form of intracellular immunity can be devised which 
      would protect cells from virus infection and, in particular, could be used as a 
      treatment for the human immunodeficiency virus (HIV) infected individual. 
      Following in vitro immunization of naive human B lymphocytes with 
      reverse-transcriptase (RT) or HIV transactivator protein (tat), messenger RNA 
      (mRNA) would be isolated from these cells. Using the mRNA molecules as templates, 
      copy DNA (cDNA) molecules encoding the RT or tat-specific immunoglobulins, are 
      prepared and amplified by the polymerase chain reaction. After engineering of the 
      antibody encoding cDNAs to provide appropriate intracellular addressing 
      information, the cDNAs would be used to transfect stem cells of HIV infected 
      individuals in vitro. The presence, in the cytoplasm and nucleus, of antibodies 
      which had been selected to interfere with the reproduction of the virus, would 
      protect these cells from infection. Autologous transplantation of such cells 
      would confer resistance against HIV replication by these stem cells and their 
      progeny in the treated individual. Such a strategy may also be useful against 
      other retroviruses and could provide resistance against retrovirally triggered 
      leukemia.
FAU - Faraji-Shadan, F
AU  - Faraji-Shadan F
AD  - Department of Biology, San Francisco State University, CA 94132.
FAU - Stubbs, J D
AU  - Stubbs JD
FAU - Bowman, P D
AU  - Bowman PD
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Med Hypotheses
JT  - Medical hypotheses
JID - 7505668
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - DNA/genetics
MH  - Genetic Engineering
MH  - Genetic Therapy/*methods
MH  - Genetic Vectors
MH  - HIV/enzymology/genetics/immunology
MH  - HIV Infections/genetics/immunology/*therapy
MH  - Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/immunology
MH  - Humans
MH  - Immunization
MH  - Models, Genetic
MH  - RNA-Directed DNA Polymerase/genetics/immunology
MH  - Transfection
MH  - Transplantation, Autologous
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
AID - 0306-9877(90)90027-C [pii]
AID - 10.1016/0306-9877(90)90027-c [doi]
PST - ppublish
SO  - Med Hypotheses. 1990 Jun;32(2):81-4. doi: 10.1016/0306-9877(90)90027-c.