PMID- 1695999
OWN - NLM
STAT- MEDLINE
DCOM- 19900905
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 113
IP  - 2
DP  - 1990 May 31
TI  - Photoconversion of fluorescent retrograde tracers.
PG  - 127-33
AB  - Photoconversion of fluorescent dyes, retrogradely transported through axons to 
      their parent cell bodies, into a stable diaminobenzidine (DAB) reaction product 
      was tested in the nigrostriatal and thalamocortical systems of rats. Satisfactory 
      results were obtained with Propidium Iodide (PI), Fluoro-Gold (FG), Fast Blue 
      (FB), Diamidino Yellow (DY), and rhodamine-labeled latex microspheres (RLM); some 
      photoconversion was also observed in Evans Blue (EB)-labeled neurons. The red 
      fluorescent tracers PI, EB and RLM were photoconverted under the excitation 
      wavelength appropriate for eliciting their fluorescent emission. With the yellow 
      or blue fluorescent tracers FG, FB, and DY satisfactory results could instead be 
      obtained using an excitation wavelength which did not elicit visible fluorescent 
      emission. This finding indicates that the latter is not a critical factor for 
      obtaining photoconversion. The features and subcellular localization of 
      photoconverted DAB were different from those of the fluorescent labeling: 
      photoconversion resulted in the appearance of brown granules of DAB reaction 
      products in the cytoplasm, independently from the occurrence of fluorescent 
      labeling in the neuronal cytoplasm or nucleus. Photoconversion may enable new 
      applications of fluorescent retrograde tracing and, in particular, its electron 
      microscopic visualization.
FAU - Bentivoglio, M
AU  - Bentivoglio M
AD  - Institute of Anatomy, Medical Faculty, University of Verona, Italy.
FAU - Su, H S
AU  - Su HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Phenanthridines)
RN  - 0 (Stilbamidines)
RN  - 36015-30-2 (Propidium)
SB  - IM
MH  - Animals
MH  - *Axonal Transport
MH  - Brain/*cytology
MH  - Corpus Striatum
MH  - *Fluorescent Dyes
MH  - Microscopy, Fluorescence/*methods
MH  - Neural Pathways/cytology
MH  - *Phenanthridines
MH  - *Propidium
MH  - Rats
MH  - *Stilbamidines
MH  - Substantia Nigra
MH  - Thalamus
EDAT- 1990/05/31 00:00
MHDA- 1990/05/31 00:01
CRDT- 1990/05/31 00:00
PHST- 1990/05/31 00:00 [pubmed]
PHST- 1990/05/31 00:01 [medline]
PHST- 1990/05/31 00:00 [entrez]
AID - 0304-3940(90)90291-G [pii]
AID - 10.1016/0304-3940(90)90291-g [doi]
PST - ppublish
SO  - Neurosci Lett. 1990 May 31;113(2):127-33. doi: 10.1016/0304-3940(90)90291-g.