PMID- 16960065 OWN - NLM STAT- MEDLINE DCOM- 20080226 LR - 20060908 IS - 1525-3198 (Electronic) IS - 0022-0302 (Linking) VI - 89 IP - 10 DP - 2006 Oct TI - Effects of dietary unsaturated fatty acids on oocyte quality and follicular development in lactating dairy cows in summer. PG - 3891-903 AB - Dietary sources of fatty acids were evaluated for their influence on oocyte quality and follicular development using 54 lactating cows in summer. Fat supplements were 1) sunflower oil (80% cis 18:1), 2) Ca salt of transoctadecenoic acids (57% trans 18:1), 3) Ca salt of vegetable oils (30% 18:2), and 4) linseed oil (56% 18:3 and 16% 18:2). Fats were fed at 1.35% of dietary dry matter beginning at 5 wk prior to expected calving date and at 1.5% (oils) and 1.75% (Ca salts) of dietary dry matter for 15 wk after parturition. Four days following a programmed induced ovulation, 5 transvaginal oocyte aspirations were performed 3 or 4 d apart. Three days after the last aspiration, PGF2alpha was injected, followed 3 d later by a GnRH injection and a timed artificial insemination (d 0) 16 to 20 h later. For the first 4 aspirations, oocytes grading 1 or 2 were used for in vitro embryo production. Total cell number and the proportion of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive blastomeres were analyzed at d 8. At the fifth aspiration, the occurrence of metaphase II, group II caspase activity, and TUNEL labeling were determined after oocyte maturation. A total of 1,011 oocytes were collected. The proportion of oocytes with high caspase activity was greater for grade 3 compared with grades 1 and 2 (37.5 vs. 1.54 and 1.61%). Feeding polyunsaturated fatty acids, as compared with monosaturated fatty acids, failed to affect oocyte quality, as demonstrated by subsequent embryo development. Cows fed 18:2- or 18:3-enriched diets had a larger preovulatory follicle at insemination and subsequent volume of the corpus luteum compared with those fed cis 18:1 or trans 18:1 diets (16.8, 16.2 vs. 15.0, 14.9 +/- 0.7 mm; 7,323, 8,208 vs. 6,033, 5,495 +/- 644 mm3, respectively). The previously documented benefits of polyunsaturated fatty acids on reproductive performance appear to reflect actions at alternative biological windows in lactating dairy cows. FAU - Bilby, T R AU - Bilby TR AD - Department of Animal Sciences, University of Florida, Gainesville, Florida 32611-0910, USA. FAU - Block, J AU - Block J FAU - do Amaral, B C AU - do Amaral BC FAU - Sa Filho, O AU - Sa Filho O FAU - Silvestre, F T AU - Silvestre FT FAU - Hansen, P J AU - Hansen PJ FAU - Staples, C R AU - Staples CR FAU - Thatcher, W W AU - Thatcher WW LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PL - United States TA - J Dairy Sci JT - Journal of dairy science JID - 2985126R RN - 0 (Dietary Fats) RN - 0 (Fatty Acids, Unsaturated) RN - 0 (Hormones) RN - 67763-96-6 (Insulin-Like Growth Factor I) RN - EC 3.4.22.- (Caspases) SB - IM MH - Animals MH - Body Temperature/drug effects MH - Caspases/metabolism MH - Cattle/embryology/*physiology MH - Dairying MH - Dietary Fats/administration & dosage/analysis/*pharmacology MH - Embryo, Mammalian/drug effects MH - Estrus/physiology MH - Fatty Acids, Unsaturated/administration & dosage/*pharmacology MH - Female MH - Fertilization in Vitro/veterinary MH - Hormones/blood MH - In Situ Nick-End Labeling/veterinary MH - Insulin-Like Growth Factor I/analysis MH - Lactation MH - Oocytes/*drug effects/physiology MH - Ovarian Follicle/*drug effects/physiology MH - Pregnancy MH - Seasons EDAT- 2006/09/09 09:00 MHDA- 2008/02/27 09:00 CRDT- 2006/09/09 09:00 PHST- 2006/09/09 09:00 [pubmed] PHST- 2008/02/27 09:00 [medline] PHST- 2006/09/09 09:00 [entrez] AID - S0022-0302(06)72432-8 [pii] AID - 10.3168/jds.S0022-0302(06)72432-8 [doi] PST - ppublish SO - J Dairy Sci. 2006 Oct;89(10):3891-903. doi: 10.3168/jds.S0022-0302(06)72432-8.