PMID- 16962081
OWN - NLM
STAT- MEDLINE
DCOM- 20061212
LR  - 20061009
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1116
IP  - 1
DP  - 2006 Oct 20
TI  - Hypertension downregulates the expression of brain-derived neurotrophic factor in 
      the ischemia-vulnerable hippocampal CA1 and cortical areas after carotid artery 
      occlusion.
PG  - 31-8
AB  - We studied the effect of hypertension on brain damage and brain-derived 
      neurotrophic factor (BDNF) expression in the hippocampal formation and cerebral 
      cortex after permanent occlusion of bilateral common carotid arteries (CCA). Two 
      groups of rats were used, including normotensive Wistar-Kyoto (WKY) rat and 
      spontaneous hypertensive rat (SHR). Each group contained sham operation, 1 week 
      and 4 weeks after bilateral CCA occlusion (n=5-10 in each time point). The blood 
      pressure showed a significant elevation in WKY rats from 1 h after operation to 4 
      weeks before sacrifice (P<0.05), but was not changed in SHR (P>0.05). However, 
      rectal temperature showed no significant change after operation in WKY rat and 
      SHR (P>0.05) and showed no significant difference at any time point between WKY 
      rat and SHR (P>0.05). Hematoxylin and eosin staining showed SHR had a 
      significantly larger necrotic volume than WKY rats (n=10 in each group, 
      6044+/-6895 microm(3) vs. 144+/-174 microm(3), P<0.05) at 4 weeks after ischemia. 
      In SHR, BDNF immunoreactivity and mRNA decreased significantly from 1 week to 4 
      weeks in both the hippocampal CA1 and cortical areas (P<0.01) but decreased 
      transiently in dentate gyrus. However, in WKY rats, BDNF immunoreactivity and 
      mRNA decreased transiently at 1 week (P<0.05) and recovered at 4 weeks after 
      cerebral ischemia. Our study demonstrates that after bilateral CCA occlusion, 
      preexisting hypertension may aggravate the brain injury and downregulate the 
      expression of BDNF immunoreactivity and mRNA in the ischemia-vulnerable 
      hippocampal CA1 and cortical areas but not in ischemia-resistant dentate gyrus.
FAU - Lee, Tsong-Hai
AU  - Lee TH
AD  - Stroke Section, Department of Neurology, Chang Gung Memorial Hospital, Linkou 
      Medical Center, College of Medicine, Chang Gung University, 5 Fu-Hsing St., 
      Kuei-Shan, Tao-Yuan, 33333 Taiwan. thlee@adm.cgmh.org.tw
FAU - Yang, Jen-Tsung
AU  - Yang JT
FAU - Kato, Hiroyuki
AU  - Kato H
FAU - Wu, June Hsieh
AU  - Wu JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060907
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain Ischemia/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis
MH  - Carotid Artery, Common/physiology
MH  - Carotid Stenosis/*metabolism
MH  - Cerebral Cortex/*metabolism
MH  - Cerebrovascular Circulation/physiology
MH  - Down-Regulation/physiology
MH  - Hippocampus/*metabolism
MH  - Hypertension/genetics/*metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Species Specificity
EDAT- 2006/09/12 09:00
MHDA- 2006/12/13 09:00
CRDT- 2006/09/12 09:00
PHST- 2005/09/01 00:00 [received]
PHST- 2006/07/17 00:00 [revised]
PHST- 2006/07/29 00:00 [accepted]
PHST- 2006/09/12 09:00 [pubmed]
PHST- 2006/12/13 09:00 [medline]
PHST- 2006/09/12 09:00 [entrez]
AID - S0006-8993(06)02301-8 [pii]
AID - 10.1016/j.brainres.2006.07.117 [doi]
PST - ppublish
SO  - Brain Res. 2006 Oct 20;1116(1):31-8. doi: 10.1016/j.brainres.2006.07.117. Epub 
      2006 Sep 7.