PMID- 16963008
OWN - NLM
STAT- MEDLINE
DCOM- 20061114
LR  - 20111117
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 241
IP  - 1
DP  - 2006 May
TI  - Identification of hepatitis C virus (HCV) 2a-derived epitope peptides having the 
      capacity to induce cytotoxic T lymphocytes in human leukocyte antigen-A24+ and 
      HCV2a-infected patients.
PG  - 38-46
AB  - Since virus-specific cytotoxic T lymphocytes (CTLs) play a critical role in 
      preventing the spread of hepatitis C virus (HCV), vaccine-based HCV-specific CTL 
      induction could be a promising strategy to treat HCV-infected patients. In this 
      study, we tried to identify HCV2a-derived epitopes, which can induce human 
      leukocyte antigen (HLA)-A24-restricted and peptide-specific CTLs. Peripheral 
      blood mononuclear cells of HCV2a-infected patients or healthy donors were 
      stimulated in vitro with HCV2a-derived peptides, which were prepared based on the 
      HLA-A24 binding motif. As a result, three peptides (HCV2a 576-584, HCV2a 627-635, 
      and HCV2a 1085-1094) efficiently induced peptide-specific CTLs from HLA-A24(+) 
      HCV2a-infected patients as well as healthy donors. The cytotoxicity was exhibited 
      by peptide-specific CD8(+) T cells in an HLA-A24-restricted manner. In addition, 
      the HCV2a 627-635 peptide was frequently recognized by immunoglobulin G of 
      HCV2a-infected patients. These results indicate that the identified three HCV2a 
      peptides might be applicable to peptide-based immunotherapy for HLA-A24(+) 
      HCV2a-infected patients.
FAU - Wang, Yi
AU  - Wang Y
AD  - Cancer Vaccine Development Division, Kurume University Research Center for 
      Innovative Cancer Therapy, 67 Asahi-machi, Kurume, Fukuoka 830-0011, Japan.
FAU - Takao, Yukari
AU  - Takao Y
FAU - Harada, Mamoru
AU  - Harada M
FAU - Komatsu, Nobukazu
AU  - Komatsu N
FAU - Ono, Takeharu
AU  - Ono T
FAU - Sata, Michio
AU  - Sata M
FAU - Itoh, Kyogo
AU  - Itoh K
FAU - Yamada, Akira
AU  - Yamada A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060911
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A24 Antigen)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cytotoxicity, Immunologic
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - HLA-A Antigens/*biosynthesis/blood
MH  - HLA-A24 Antigen
MH  - Hepacivirus/*immunology
MH  - Hepatitis C, Chronic/immunology/metabolism
MH  - Humans
MH  - Lymphocyte Activation/immunology
MH  - Peptide Fragments/*immunology
MH  - T-Lymphocyte Subsets/immunology/virology
MH  - T-Lymphocytes, Cytotoxic/*immunology/*virology
EDAT- 2006/09/12 09:00
MHDA- 2006/11/15 09:00
CRDT- 2006/09/12 09:00
PHST- 2006/06/06 00:00 [received]
PHST- 2006/07/24 00:00 [revised]
PHST- 2006/07/28 00:00 [accepted]
PHST- 2006/09/12 09:00 [pubmed]
PHST- 2006/11/15 09:00 [medline]
PHST- 2006/09/12 09:00 [entrez]
AID - S0008-8749(06)00130-4 [pii]
AID - 10.1016/j.cellimm.2006.07.009 [doi]
PST - ppublish
SO  - Cell Immunol. 2006 May;241(1):38-46. doi: 10.1016/j.cellimm.2006.07.009. Epub 
      2006 Sep 11.