PMID- 16963665
OWN - NLM
STAT- MEDLINE
DCOM- 20061006
LR  - 20191210
IS  - 0012-3692 (Print)
IS  - 0012-3692 (Linking)
VI  - 130
IP  - 3
DP  - 2006 Sep
TI  - A comparison of cytology and fluorescence in situ hybridization for the detection 
      of lung cancer in bronchoscopic specimens.
PG  - 694-701
AB  - STUDY OBJECTIVES: To determine the relative sensitivity and specificity of 
      cytology and fluorescence in situ hybridization (FISH) for the detection of lung 
      cancer in bronchoscopically obtained specimens. DESIGN: Cytology and FISH were 
      performed on brushing and washing specimens obtained from patients undergoing 
      bronchoscopy for suspected lung cancer. FISH utilized the LAVysion probe set 
      (Abbott Molecular; Des Plaines, IL), which contains locus-specific probes to 
      5p15, 7p12 (EGFR), 8q24 (C-MYC), and a centromeric probe to chromosome 6. 
      SETTING: Single-center, academic, tertiary medical center. PARTICIPANTS: One 
      hundred thirty-seven patients referred for bronchoscopy for suspicion of lung 
      cancer. INTERVENTIONS: Cytology and FISH were performed on bronchoscopic 
      brushings and washings. MEASUREMENTS AND RESULTS: One hundred thirty-seven 
      patients undergoing bronchoscopy had pathology, FISH, and cytology results. FISH 
      and cytology were performed on 123 washing and 78 brushing specimens. 
      Sensitivities of FISH and cytology were 71% and 51% (p = 0.007), respectively, 
      for brushing specimens, and 49% and 44% (p = 0.541) for washing specimens. When 
      FISH and cytology results were combined, sensitivities were 75% and 61%, 
      respectively, for brushing and washing specimens, which was significantly better 
      (p < 0.001) than cytology alone. Specificities of FISH and cytology for patients 
      with negative findings at the time of initial bronchoscopy were 83% and 100% (p = 
      0.125), respectively, for brushing specimens, and 95% and 100% (p = 0.500) for 
      washing specimens. CONCLUSIONS: These findings show that FISH is significantly 
      more sensitive than conventional cytology for detecting lung cancer in bronchial 
      brushing specimens; when combined with cytology, FISH can improve the diagnostic 
      sensitivity of detecting malignancy in bronchial brushing and washing specimens.
FAU - Halling, Kevin C
AU  - Halling KC
AD  - Department of Laboratory Medicine, Mayo Clinic, Rochester, MN 55905, USA. 
      halling.kevin@mayo.edu
FAU - Rickman, Otis B
AU  - Rickman OB
FAU - Kipp, Benjamin R
AU  - Kipp BR
FAU - Harwood, Aaron R
AU  - Harwood AR
FAU - Doerr, Clinton H
AU  - Doerr CH
FAU - Jett, James R
AU  - Jett JR
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Chest
JT  - Chest
JID - 0231335
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
CIN - Chest. 2006 Sep;130(3):634-5. PMID: 16963653
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bronchi/*pathology
MH  - Bronchoalveolar Lavage
MH  - Bronchoscopy
MH  - Chromosome Aberrations
MH  - Cytodiagnosis/methods
MH  - DNA Probes
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lung Neoplasms/*diagnosis/genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
MH  - Sensitivity and Specificity
EDAT- 2006/09/12 09:00
MHDA- 2006/10/07 09:00
CRDT- 2006/09/12 09:00
PHST- 2006/09/12 09:00 [pubmed]
PHST- 2006/10/07 09:00 [medline]
PHST- 2006/09/12 09:00 [entrez]
AID - S0012-3692(15)52781-5 [pii]
AID - 10.1378/chest.130.3.694 [doi]
PST - ppublish
SO  - Chest. 2006 Sep;130(3):694-701. doi: 10.1378/chest.130.3.694.