PMID- 16963754
OWN - NLM
STAT- MEDLINE
DCOM- 20061026
LR  - 20201006
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 87
IP  - Pt 10
DP  - 2006 Oct
TI  - T-cell responses to peptide fragments of the BK virus T antigen: implications for 
      cross-reactivity of immune response to JC virus.
PG  - 2951-2960
LID - 10.1099/vir.0.82094-0 [doi]
AB  - Infection with BK virus (BKV) induces both humoral and cellular immunity, but the 
      viral antigens of T-antigen (T-ag) stimulating T-cell responses are largely 
      unknown. To identify BKV-specific T cells in healthy individuals, peripheral 
      blood lymphocytes were cultured with autologous dendritic cells (DCs) loaded with 
      BKV lysate and T cells were screened for intracellular gamma interferon 
      production after stimulation with an overlapping 15mer peptide library of the BKV 
      T-ag. Among many immunogenic peptides identified, four T-ag peptides were 
      identified as candidate major histocompatibility complex class I and II T-cell 
      epitopes, restricted to human leukocyte antigen (HLA)-B*0702, -B*08, -DRB1*0301 
      and -DRB1*0901. Further, a candidate 9mer peptide, LPLMRKAYL, was confirmed to be 
      restricted to HLA-B*0702 and -B*08. Because the polyomaviruses BKV, JC virus 
      (JCV) and Simian virus 40 (SV40) share extensive sequence similarity in the 
      immunogenic proteins T-ag and VP1, it was hypothesized that, in humans, these 
      proteins contain conserved cytotoxic T-lymphocyte (CTL) target epitopes. Four 
      HLA-restricted conserved epitopes of BKV, JCV and SV40 were identified: HLA-B*07, 
      -B*08 and -DRB1*0901 for T-ag and -A*0201 for VP1. T cells cultured in vitro that 
      were specific for one viral antigen recognized other conserved epitopes. CTLs 
      generated from BKV T-ag and VP1 peptide were cytotoxic to DC targets pulsed with 
      either BKV or JCV. Therefore, infection by one of the two viruses (BKV and JCV) 
      could establish cross-immunity against the other. Although cross-cytotoxicity 
      experiments were not performed with SV40, cross-recognition data from conserved 
      antigen epitopes of polyomaviruses suggest strongly that cross-immunity might 
      also exist among the three viruses.
FAU - Li, Jongming
AU  - Li J
AD  - Medical Oncology Division, Thomas Jefferson University, Philadelphia, PA, USA.
FAU - Melenhorst, Jos
AU  - Melenhorst J
AD  - Hematology Branch, National Heart, Lung, Blood Institute, National Institutes of 
      Health, Bldg 10 CRC, Room 3-5330, 10 Center Drive, Bethesda, MD 20892-1202, USA.
FAU - Hensel, Nancy
AU  - Hensel N
AD  - Hematology Branch, National Heart, Lung, Blood Institute, National Institutes of 
      Health, Bldg 10 CRC, Room 3-5330, 10 Center Drive, Bethesda, MD 20892-1202, USA.
FAU - Rezvani, Katyoun
AU  - Rezvani K
AD  - Hematology Branch, National Heart, Lung, Blood Institute, National Institutes of 
      Health, Bldg 10 CRC, Room 3-5330, 10 Center Drive, Bethesda, MD 20892-1202, USA.
FAU - Sconocchia, Giuseppe
AU  - Sconocchia G
AD  - Hematology Branch, National Heart, Lung, Blood Institute, National Institutes of 
      Health, Bldg 10 CRC, Room 3-5330, 10 Center Drive, Bethesda, MD 20892-1202, USA.
FAU - Kilical, Yasemin
AU  - Kilical Y
AD  - Hematology Branch, National Heart, Lung, Blood Institute, National Institutes of 
      Health, Bldg 10 CRC, Room 3-5330, 10 Center Drive, Bethesda, MD 20892-1202, USA.
FAU - Hou, Jean
AU  - Hou J
AD  - Laboratory of Molecular Medicine and Neuroscience, National Institute of 
      Neurological Disorders, National Institutes of Health, Bethesda, MD, USA.
FAU - Curfman, Blanche
AU  - Curfman B
AD  - Laboratory of Molecular Medicine and Neuroscience, National Institute of 
      Neurological Disorders, National Institutes of Health, Bethesda, MD, USA.
FAU - Major, Eugene
AU  - Major E
AD  - Laboratory of Molecular Medicine and Neuroscience, National Institute of 
      Neurological Disorders, National Institutes of Health, Bethesda, MD, USA.
FAU - Barrett, A John
AU  - Barrett AJ
AD  - Hematology Branch, National Heart, Lung, Blood Institute, National Institutes of 
      Health, Bldg 10 CRC, Room 3-5330, 10 Center Drive, Bethesda, MD 20892-1202, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes, T-Lymphocyte)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Viral/*chemistry/*immunology
MH  - BK Virus/*immunology
MH  - Cross Reactions/immunology
MH  - Epitopes, T-Lymphocyte/immunology
MH  - Humans
MH  - JC Virus/*immunology
MH  - Simian virus 40/immunology
MH  - T-Lymphocytes/*immunology
EDAT- 2006/09/12 09:00
MHDA- 2006/10/27 09:00
CRDT- 2006/09/12 09:00
PHST- 2006/09/12 09:00 [pubmed]
PHST- 2006/10/27 09:00 [medline]
PHST- 2006/09/12 09:00 [entrez]
AID - 10.1099/vir.0.82094-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2006 Oct;87(Pt 10):2951-2960. doi: 10.1099/vir.0.82094-0.