PMID- 16965157
OWN - NLM
STAT- MEDLINE
DCOM- 20070104
LR  - 20191210
IS  - 1083-3668 (Print)
IS  - 1083-3668 (Linking)
VI  - 11
IP  - 4
DP  - 2006 Jul-Aug
TI  - Linear response range characterization and in vivo application of laser speckle 
      imaging of blood flow dynamics.
PG  - 041129
AB  - Noninvasive blood flow imaging can provide critical information on the state of 
      biological tissue and the efficacy of approaches to treat disease. With laser 
      speckle imaging (LSI), relative changes in blood flow are typically reported, 
      with the assumption that the measured values are on a linear scale. A linear 
      relationship between the measured and actual flow rate values has been suggested. 
      The actual flow rate range, over which this linear relationship is valid, is 
      unknown. Herein we report the linear response range and velocity dynamic range 
      (VDR) of our LSI instrument at two relevant camera integration times. For 
      integration times of 1 and 10 ms, the best case VDR was 80 and 60 dB, 
      respectively, and the worst case VDR was 20 and 50 dB. The best case VDR values 
      were similar to those reported in the literature for optical Doppler tomography. 
      We also demonstrate the potential of LSI for monitoring blood flow dynamics in 
      the rodent dorsal skinfold chamber model. These findings imply that LSI can 
      provide accurate wide-field maps of microvascular blood flow rate dynamics and 
      highlight heterogeneities in flow response to the application of exogenous 
      agents.
FAU - Choi, Bernard
AU  - Choi B
AD  - University of California, Irvine, Beckman Laser Institute and Medical Clinic, 
      1002 Health Sciences Road East, Irvine, California 92612, USA. choib@uci.edu
FAU - Ramirez-San-Juan, Julio C
AU  - Ramirez-San-Juan JC
FAU - Lotfi, Justin
AU  - Lotfi J
FAU - Stuart Nelson, J
AU  - Stuart Nelson J
LA  - eng
GR  - AR047551/AR/NIAMS NIH HHS/United States
GR  - AR048458/AR/NIAMS NIH HHS/United States
GR  - EB02495/EB/NIBIB NIH HHS/United States
GR  - P41-RR01195/RR/NCRR NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Opt
JT  - Journal of biomedical optics
JID - 9605853
SB  - IM
MH  - Animals
MH  - Blood Flow Velocity/*physiology
MH  - Image Interpretation, Computer-Assisted/*methods
MH  - *Lasers
MH  - Linear Models
MH  - Microscopy, Confocal/*methods
MH  - Models, Cardiovascular
MH  - Phantoms, Imaging
MH  - Rats
MH  - Reproducibility of Results
MH  - Rheology/*methods
MH  - Sensitivity and Specificity
MH  - Skin/*blood supply
EDAT- 2006/09/13 09:00
MHDA- 2007/01/05 09:00
CRDT- 2006/09/13 09:00
PHST- 2006/09/13 09:00 [pubmed]
PHST- 2007/01/05 09:00 [medline]
PHST- 2006/09/13 09:00 [entrez]
AID - 10.1117/1.2341196 [doi]
PST - ppublish
SO  - J Biomed Opt. 2006 Jul-Aug;11(4):041129. doi: 10.1117/1.2341196.