PMID- 16965978
OWN - NLM
STAT- MEDLINE
DCOM- 20061205
LR  - 20131121
IS  - 0301-5629 (Print)
IS  - 0301-5629 (Linking)
VI  - 32
IP  - 9
DP  - 2006 Sep
TI  - Effects of simultaneous application of ultrasound and microbubbles on 
      intracerebral hemorrhage in an animal model.
PG  - 1377-82
AB  - Microbubble-enhanced sonothrombolysis (MEST) may be an alternative therapeutic 
      option in ischemic stroke. Clinical study of the efficacy of MEST as an adjunct 
      stroke therapy, before imaging with CT or MRI, requires experimental data on the 
      safety of this approach in the presence of hemorrhagic stroke. We, therefore, 
      investigated the effect of diagnostic transcranial ultrasound combined with 
      microbubbles (US + MB) in an experimental animal model of intracerebral 
      hemorrhage (ICH). ICH was induced in anesthetized rats by intracerebral 
      collagenase injection. Transcranial ultrasound (2 MHz, mechanical index 1.3, 1051 
      kPa) was applied 3 h after ICH induction to rat brains for 30 min during a 
      continuous IV infusion of sulfur hexafluoride microbubbles (SonoVue). The size of 
      cerebral hemorrhage, the extent of brain edema, and the amount of apoptosis were 
      compared with those from control rats with ICH but without US + MB. Results 
      showed no significant effect of US + MB on hemorrhage size (control 23.3 +/- 10.7 
      mm(3), US + MB 20.3 +/- 5.8 mm(3)), on the extent of brain edema (control 3.3 +/- 
      2.0%, US +MB 3.5 +/- 1.9%), or on the rate of apoptosis (control 5.2 +/- 1.5%, US 
      + MB 5.2 +/- 1.0%). We conclude that diagnostic ultrasound in combination with 
      microbubbles does not cause additional damage to the rat brain during ICH in our 
      experimental set-up. This finding provides support for the use of MEST as an 
      early stroke therapy.
FAU - Stroick, Mark
AU  - Stroick M
AD  - Department of Neurology, Universitatsklinikum Mannheim, University of Heidelberg, 
      Mannheim, Germany.
FAU - Alonso, Angelika
AU  - Alonso A
FAU - Fatar, Marc
AU  - Fatar M
FAU - Griebe, Martin
AU  - Griebe M
FAU - Kreisel, Stefan
AU  - Kreisel S
FAU - Kern, Rolf
AU  - Kern R
FAU - Gaud, Emmanuel
AU  - Gaud E
FAU - Arditi, Marcel
AU  - Arditi M
FAU - Hennerici, Michael
AU  - Hennerici M
FAU - Meairs, Stephen
AU  - Meairs S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Ultrasound Med Biol
JT  - Ultrasound in medicine & biology
JID - 0410553
RN  - 0 (Contrast Media)
RN  - 0 (Phospholipids)
RN  - 0 (contrast agent BR1)
RN  - WS7LR3I1D6 (Sulfur Hexafluoride)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Brain Edema/etiology/pathology
MH  - Cerebral Hemorrhage/*complications/pathology
MH  - Contrast Media/adverse effects
MH  - Disease Models, Animal
MH  - Male
MH  - Microbubbles/*adverse effects
MH  - Phospholipids/*adverse effects
MH  - Rats
MH  - Rats, Wistar
MH  - Stroke/complications/therapy
MH  - Sulfur Hexafluoride/*adverse effects
MH  - Ultrasonic Therapy/*adverse effects
EDAT- 2006/09/13 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/09/13 09:00
PHST- 2005/08/12 00:00 [received]
PHST- 2006/04/28 00:00 [revised]
PHST- 2006/05/11 00:00 [accepted]
PHST- 2006/09/13 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/09/13 09:00 [entrez]
AID - S0301-5629(06)01626-7 [pii]
AID - 10.1016/j.ultrasmedbio.2006.05.027 [doi]
PST - ppublish
SO  - Ultrasound Med Biol. 2006 Sep;32(9):1377-82. doi: 
      10.1016/j.ultrasmedbio.2006.05.027.