PMID- 16966142
OWN - NLM
STAT- MEDLINE
DCOM- 20061031
LR  - 20071114
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 31
IP  - 9
DP  - 2006 Sep
TI  - Apolipoprotein E modulates establishment of HSV-1 latency and survival in a mouse 
      ocular model.
PG  - 703-8
AB  - PURPOSE: To evaluate and compare the neuroinvasiveness and neurovirulence after 
      ocular HSV-1 infection in ApoE knockout (ApoE-/-) and control C57BL/6 (ApoE+/+) 
      mice. METHODS: Age-matched (14 weeks of age) C57BL/6J (ApoE+/+) female mice and 
      female ApoE knockout (ApoE-/-) mice were inoculated by corneal scarification with 
      HSV-1 strain 17Syn+. Analysis of HSV-1 replication in the mouse cornea was 
      assessed through infectious virus assays of ocular (tear film) swabs at 1 to 5 
      days postinoculation (PI), slit-lamp examination (SLE) of corneas at PI days 1 to 
      7, and survival of infected mice. The contribution of apoE to the efficient 
      establishment of latency was measured by real-time PCR quantitation of the latent 
      viral genome in the trigeminal ganglia (TG) of infected mice. RESULTS: These 
      studies showed that HSV-1 strain 17Syn+ replicates efficiently in the eyes, 
      regardless of the host ApoE genotype. Neither the scoring of corneal pathology 
      via SLE nor the infectious virus assay of the tear film resulted in any 
      statistical differences between ApoE knockout (-/-) mice or the C57BL/6 (ApoE+/+) 
      mice. In mice latently infected with HSV-1, our real-time PCR data showed 
      significantly lower viral copy numbers of HSV-1 DNA in ApoE knockout (ApoE-/-) 
      mice compared with C57BL/6 (ApoE+/+) mice. C57BL/6 (ApoE+/+) mice are more 
      susceptible to the neurovirulence of HSV-1 strain 17Syn+ than female ApoE 
      knockout (-/-) mice, as demonstrated by the fact that 50% (7/14) of the female 
      C57BL/6 (ApoE+/+) mice inoculated with 17Syn+ died, as opposed to none (0/14) of 
      the age- and sex-matched ApoE knockout mice. CONCLUSIONS: These data indicate 
      that age (14 weeks) and sex-matched (female) wild mice with an ApoE null 
      background (ApoE-/-) are more resistant and less efficient in the establishment 
      of latency compared with ApoE+/+ mice in the C57BL/6 background.
FAU - Bhattacharjee, Partha S
AU  - Bhattacharjee PS
AD  - Department of Ophthalmology, Louisiana State University Health Sciences Center, 
      New Orleans, Louisiana, USA.
FAU - Neumann, Donna M
AU  - Neumann DM
FAU - Stark, David
AU  - Stark D
FAU - Thompson, Hilary W
AU  - Thompson HW
FAU - Hill, James M
AU  - Hill JM
LA  - eng
GR  - 2P20RR016456-04A1/RR/NCRR NIH HHS/United States
GR  - 5F32EY016316/EY/NEI NIH HHS/United States
GR  - EY006311/EY/NEI NIH HHS/United States
GR  - EY02377/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Apolipoproteins E)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Apolipoproteins E/*physiology
MH  - Cornea/virology
MH  - DNA, Viral/analysis
MH  - Disease Models, Animal
MH  - Female
MH  - Genome, Viral
MH  - Herpesvirus 1, Human/pathogenicity/*physiology
MH  - Keratitis, Herpetic/mortality/*virology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Polymerase Chain Reaction
MH  - Survival Rate
MH  - Virulence
MH  - Virus Latency/*physiology
MH  - Virus Replication/physiology
EDAT- 2006/09/13 09:00
MHDA- 2006/11/01 09:00
CRDT- 2006/09/13 09:00
PHST- 2006/09/13 09:00 [pubmed]
PHST- 2006/11/01 09:00 [medline]
PHST- 2006/09/13 09:00 [entrez]
AID - LX05150150361238 [pii]
AID - 10.1080/02713680600864600 [doi]
PST - ppublish
SO  - Curr Eye Res. 2006 Sep;31(9):703-8. doi: 10.1080/02713680600864600.