PMID- 16966220
OWN - NLM
STAT- MEDLINE
DCOM- 20061214
LR  - 20181113
IS  - 1355-0284 (Print)
IS  - 1538-2443 (Electronic)
IS  - 1355-0284 (Linking)
VI  - 12
IP  - 4
DP  - 2006 Aug
TI  - Cerebrospinal fluid is an efficient route for establishing brain infection with 
      feline immunodeficiency virus and transfering infectious virus to the periphery.
PG  - 294-306
AB  - Like human immunodeficiency virus (HIV), feline immunodeficiency virus (FIV) 
      invades and infects the central nervous system (CNS) soon after peripheral 
      infection. The appearance of viral RNA is particularly prominent in the 
      cerebrospinal fluid (CSF), suggesting an efficient route of virus transfer across 
      the blood-CSF barrier. This raises the concern whether this route can establish a 
      stable viral reservoir and also be a source of virus capable of reseeding 
      peripheral systems. To examine this possibility, 200 mul of cell-free NCSU1 FIV 
      or FIV-infected choroid plexus macrophages (ChP-Mac) was directly injected into 
      the right lateral ventricle of the brain. Negative controls were sham inoculated 
      with uninfected ChP-Mac or virus-free culture supernatant and positive controls 
      were infected systemically by intraperitoneal (i.p.) injection. 
      Intracerebroventricular (i.c.v.) inoculation with cell-free FIV resulted in high 
      levels of plasma FIV RNA detected as early as 1 to 2 weeks post inoculation in 
      all cats. In each case, the plasma viremia preceded the detection of CSF viral 
      RNA. Compared to i.p. cats, i.c.v. cats had 32-fold higher CSF viral loads, 
      8-fold higher ratios of CSF to plasma viral load, and a 23-fold greater content 
      of FIV proviral DNA in the brain. No FIV RNA was detected in plasma or CSF from 
      the cats inoculated with FIV-infected ChP-Mac but an acute inflammatory response 
      and a slight suppression of the CD4+:CD8+ ratio were observed. These results 
      indicate that free FIV circulating in the CSF promotes infection of the CNS and 
      provides a highly efficient pathway for the transfer of infectious virus to the 
      periphery.
FAU - Liu, Pinghuang
AU  - Liu P
AD  - Immunology Program, College of Veterinary Medicine, North Carolina State 
      University, Raleigh, North Carolina, USA.
FAU - Hudson, Lola C
AU  - Hudson LC
FAU - Tompkins, Mary B
AU  - Tompkins MB
FAU - Vahlenkamp, Thomas W
AU  - Vahlenkamp TW
FAU - Colby, Brenda
AU  - Colby B
FAU - Rundle, Cyndi
AU  - Rundle C
FAU - Meeker, Rick B
AU  - Meeker RB
LA  - eng
GR  - R01 MH063646/MH/NIMH NIH HHS/United States
GR  - R01 MH063646-02/MH/NIMH NIH HHS/United States
GR  - MH63646/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Neurovirol
JT  - Journal of neurovirology
JID - 9508123
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Brain Diseases/*cerebrospinal fluid/immunology/*virology
MH  - Cats
MH  - DNA, Viral/isolation & purification
MH  - Feline Acquired Immunodeficiency Syndrome/*cerebrospinal 
      fluid/immunology/*virology
MH  - Immunodeficiency Virus, Feline/genetics/*isolation & purification
MH  - Lymphocyte Subsets/immunology
MH  - Macrophages/immunology/virology
MH  - RNA, Viral/blood/cerebrospinal fluid
MH  - Viral Load
PMC - PMC3166823
MID - NIHMS306042
EDAT- 2006/09/13 09:00
MHDA- 2006/12/15 09:00
PMCR- 2011/09/05
CRDT- 2006/09/13 09:00
PHST- 2006/09/13 09:00 [pubmed]
PHST- 2006/12/15 09:00 [medline]
PHST- 2006/09/13 09:00 [entrez]
PHST- 2011/09/05 00:00 [pmc-release]
AID - Q03055W1N2VL5124 [pii]
AID - 10.1080/13550280600889567 [doi]
PST - ppublish
SO  - J Neurovirol. 2006 Aug;12(4):294-306. doi: 10.1080/13550280600889567.