PMID- 1696633
OWN - NLM
STAT- MEDLINE
DCOM- 19900914
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 64
IP  - 9
DP  - 1990 Sep
TI  - Altered mouse mammary tumor virus transcript synthesis in T-cell lymphoma cells.
PG  - 4043-50
AB  - Proviral copies of mouse mammary tumor virus (MMTV) are known to be amplified in 
      certain T-cell lymphomas. Transcription of the amplified MMTV proviruses was 
      studied in detail in two T-cell lymphoma lines and showed the production of 
      deletions and premature termination of env mRNAs and the premature termination of 
      gag transcripts. EL-4 cells produce three env mRNAs, and sequence analysis of 
      cDNAs of the two smaller transcripts revealed large deletions encompassing the 3' 
      half of the env gene. The deletion in at least one of the altered transcripts 
      appeared to be produced by a splicing mechanism. T-cell lymphoma line ML of DBA/2 
      mice also synthesizes two smaller env transcripts, both of which result from 
      premature termination of transcription. Both lines transcribe high levels of gag 
      mRNAs of about 0.8 kilobases in length, terminating at the end of the region 
      encoding MMTV phosphoprotein pp21. Restriction enzyme BamHI analysis of the 
      amplified proviruses of EL-4 and ML cells as well as of additional non-mammary 
      tumor cell types containing amplified MMTV proviruses suggested that the 
      amplified proviruses were derived from exogenous viruses, or activated endogenous 
      provirus MTV-1 in the case of DBA/2 strain tumor cells.
FAU - Racevskis, J
AU  - Racevskis J
AD  - Department of Oncology, Montefiore Medical Center, Bronx, New York.
LA  - eng
SI  - GENBANK/M55694
SI  - GENBANK/M55695
SI  - GENBANK/M55696
SI  - GENBANK/M55697
GR  - CA-43864/CA/NCI NIH HHS/United States
GR  - P30 CA-13330/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, gag)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 24937-83-5 (Poly A)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Base Composition
MH  - Base Sequence
MH  - Cell Line
MH  - DNA Probes
MH  - DNA, Viral/genetics
MH  - Female
MH  - Gene Library
MH  - Gene Products, gag/genetics
MH  - Genes, gag
MH  - Lymphoma
MH  - Male
MH  - Mammary Glands, Animal/microbiology
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred DBA
MH  - Molecular Sequence Data
MH  - Poly A/genetics
MH  - Polymerase Chain Reaction
MH  - Proviruses/genetics
MH  - RNA/genetics
MH  - RNA, Messenger
MH  - RNA, Viral/genetics/isolation & purification
MH  - Restriction Mapping
MH  - T-Lymphocytes
MH  - *Transcription, Genetic
PMC - PMC247865
EDAT- 1990/09/01 00:00
MHDA- 1990/09/01 00:01
PMCR- 1990/09/01
CRDT- 1990/09/01 00:00
PHST- 1990/09/01 00:00 [pubmed]
PHST- 1990/09/01 00:01 [medline]
PHST- 1990/09/01 00:00 [entrez]
PHST- 1990/09/01 00:00 [pmc-release]
AID - 10.1128/JVI.64.9.4043-4050.1990 [doi]
PST - ppublish
SO  - J Virol. 1990 Sep;64(9):4043-50. doi: 10.1128/JVI.64.9.4043-4050.1990.