PMID- 1696701
OWN - NLM
STAT- MEDLINE
DCOM- 19900918
LR  - 20190726
IS  - 0028-3908 (Print)
IS  - 0028-3908 (Linking)
VI  - 29
IP  - 7
DP  - 1990 Jul
TI  - Reduction of in vivo binding of [3H]paroxetine in mouse brain by 
      3,4-methylenedioxymethamphetamine.
PG  - 633-9
AB  - The effects of 3,4-methylenedioxymethamphetamine (MDMA) on the in vivo binding of 
      [3H]paroxetine, a potent and selective 5-hydroxytryptamine (5-HT; serotonin) 
      uptake inhibitor, in the brain of the mouse were studied. The distribution of 
      radioactivity in the brain of the mouse, after intravenous administration of 
      [3H]paroxetine, was significantly altered by pretreatment with MDMA (15 mg/kg, 
      i.p., 3 hr before). The hypothalamus/cerebellum and cerebral cortex/cerebellum 
      ratios, as a function of time, were significantly decreased after the 
      pretreatment with MDMA, indicating that the in vivo binding of [3H]paroxetine to 
      uptake sites for 5-HT in the brain of the mouse was significantly decreased by 
      MDMA. These ratios could reflect those of the total binding, to the non-specific 
      binding and free ligand, since the cerebellum has very low levels of binding for 
      [3H]paroxetine. Furthermore, these ratios decreased after pretreatment with MDMA, 
      in a dose-dependent manner. However, the binding of [3H]paroxetine to membranes 
      from the brain of the mouse in vitro was not altered by treatment with MDMA. The 
      discrepancy between the in vivo binding and in vitro binding of [3H]paroxetine in 
      the brain of the mouse is discussed.
FAU - Hashimoto, K
AU  - Hashimoto K
AD  - Department of Radiopharmaceutical Chemistry, Faculty of Pharmacy and 
      Pharmaceutical Sciences, University of Fukuyama, Japan.
FAU - Goromaru, T
AU  - Goromaru T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (Amphetamines)
RN  - 0 (Piperidines)
RN  - 0 (Serotonin Antagonists)
RN  - 333DO1RDJY (Serotonin)
RN  - 41VRH5220H (Paroxetine)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - 54-16-0 (Hydroxyindoleacetic Acid)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/analogs & derivatives/*pharmacology
MH  - Amphetamines/*pharmacology
MH  - Analysis of Variance
MH  - Animals
MH  - Brain/*metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Thin Layer
MH  - Hydroxyindoleacetic Acid/analysis
MH  - Injections, Intravenous
MH  - Male
MH  - Mice
MH  - N-Methyl-3,4-methylenedioxyamphetamine
MH  - Paroxetine
MH  - Piperidines/*metabolism/pharmacology
MH  - Serotonin/analysis
MH  - Serotonin Antagonists/*metabolism/pharmacology
EDAT- 1990/07/01 00:00
MHDA- 1990/07/01 00:01
CRDT- 1990/07/01 00:00
PHST- 1990/07/01 00:00 [pubmed]
PHST- 1990/07/01 00:01 [medline]
PHST- 1990/07/01 00:00 [entrez]
AID - 10.1016/0028-3908(90)90024-l [doi]
PST - ppublish
SO  - Neuropharmacology. 1990 Jul;29(7):633-9. doi: 10.1016/0028-3908(90)90024-l.