PMID- 16967087
OWN - NLM
STAT- MEDLINE
DCOM- 20061030
LR  - 20171116
IS  - 1642-431X (Print)
IS  - 1642-431X (Linking)
VI  - 6 Suppl 1
DP  - 2006
TI  - Genetical and biotechnological methods of utilization of female reproductive 
      potential in mammals.
PG  - 21-36
AB  - The investigations included: 1/ Establishment of culture systems that would 
      maintain the three-dimensional structure of bovine intact early antral follicles 
      (EAF) or isolated cumulus-oocyte-granulosa complexes (COCGs) and increase the 
      resulting portion of cumulus-oocyte complexes (COCs) with normal morphology for 
      subsequent in vitro maturation (IVM) and in vitro fertilization (IVF), 2/ Quality 
      assessment of IVM bovine oocytes and resulting day-8 blastocysts produced in 
      TCM199 medium supplemented with fetal bovine serum (FBS), fatty acid free bovine 
      serum albumin (fafBSA) or polyvinylpyrrolidone (PVP40), 3/ Testing the 
      polymorphism of the genes: retinol binding protein (RBP4), epidermal growth 
      factor (EGF), prostaglandin-endoperoxide synthase 2 (PTGS2), insulin-like growth 
      factor 2 (IGF2), amphiregulin (AREG) and prolactin (PRL), and their effects on 
      reproductive traits in swine. Isolated COCGs created in culture follicle-like 
      structures and their oocytes achieved meiotic competence and matured to metaphase 
      II at a higher rate than did oocytes from smaller diameter follicles which were 
      cultured intact. The proportion of COCs with normal morphology significantly 
      increased when isolated COCGs were embedded in microdrops of collagen gel or 
      cultured on inserts covered with gel rather than in large gel volumes. No 
      significant effect of maturation media composition on meiotic spindle morphology 
      and the rate of apoptotic bovine oocytes was observed. Among day-8 embryos 
      derived from oocytes matured with PVP40 a reduced blastocyst rate and elevated 
      apoptotic index were found, whereas total cell count was not affected. Gene 
      expression study also revealed a decrease in relative abundance for IGF2 and its 
      receptor (IGF2R), and heat shock protein 70 (Hsp70) genes in PVP40 group and a 
      significant elevation in fafBSA derived embryos. The significant effect of 
      reproduction traits of swine (litter size and litter weight) was found for RBP4, 
      EGF, IGF2 and AREG genes. A new polymorphism was also revealed within a promoter 
      region of PRL gene.
FAU - Katska-Ksiazkiewicz, Lucyna
AU  - Katska-Ksiazkiewicz L
AD  - Department of Biotechnology of Animal Reproduction, National Research Institute 
      of Animal Production, Balice/Krakow, Krakowska 1, 32-083 Balice, Poland. 
      lkatska@izoo.krakow.pl
FAU - Lechniak-Cieslak, Dorota
AU  - Lechniak-Cieslak D
FAU - Korwin-Kossakowska, Agnieszka
AU  - Korwin-Kossakowska A
FAU - Alm, Hannelore
AU  - Alm H
FAU - Rynska, Bozenna
AU  - Rynska B
FAU - Warzych, Ewelina
AU  - Warzych E
FAU - Sosnowski, Jaroslaw
AU  - Sosnowski J
FAU - Sender, Grazyna
AU  - Sender G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Poland
TA  - Reprod Biol
JT  - Reproductive biology
JID - 101160559
RN  - 0 (Culture Media)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 9002-62-4 (Prolactin)
RN  - FZ989GH94E (Povidone)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Culture Media
MH  - Female
MH  - Mammals/*physiology
MH  - *Oocytes
MH  - Ovarian Follicle
MH  - Polymorphism, Genetic
MH  - Povidone
MH  - Prolactin/genetics
MH  - Reproduction/genetics
MH  - Serum Albumin, Bovine
RF  - 48
EDAT- 2006/09/13 09:00
MHDA- 2006/10/31 09:00
CRDT- 2006/09/13 09:00
PHST- 2006/09/13 09:00 [pubmed]
PHST- 2006/10/31 09:00 [medline]
PHST- 2006/09/13 09:00 [entrez]
PST - ppublish
SO  - Reprod Biol. 2006;6 Suppl 1:21-36.