PMID- 169673
OWN - NLM
STAT- MEDLINE
DCOM- 19751126
LR  - 20161123
IS  - 0386-300X (Print)
IS  - 0386-300X (Linking)
VI  - 29
IP  - 2
DP  - 1975 Apr
TI  - Undifferentiated patterns of key glycolytic and gluconeogenic enzymes in 
      epithelial cell lines derived from rat liver.
PG  - 151-9
AB  - Established epithelial cell lines derived from livers of 7-day (B, B-R, B-3-4-7, 
      J-C-1, J-C-13, J-5-2-1, E-C-4 and E-C-7) and adult (AL-2, AL-3, AL-4, AL-5 and 
      AL-6) rats were analyzed for hexokinase (HK), pyruvate kinase (PK), glucose 
      6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), 
      glucose 6-phosphatase (G6Pase) and fructose 1,6-diphosphatase (FDPase). None of 
      the cell lines showed appreciable activities of adult type liver enzymes (HK Type 
      IVs (glucokinase), PK Type L, G6Pase and FDPase). On the contrary, the activities 
      of fetal type liver enzymes (HK Types I and II, PK Type M2 and G6PD) increased 
      markedly as compared with dispersed cells or tissues of adult liver. 6PGD gave 
      minimum changes in activity, and the 6PGD/G6PD ratio decreased consistently. HK 
      Type III was found only in J-C-13, AL-5 and AL-6, while HK Type IVf (high Km) was 
      present in all the cell lines examined. Possible explanations for the 
      undifferentiated patterns of carbohydrate-metabolizing enzymes in the established 
      cell lines, which have several evidence of hepatocyte origin, are presented.
FAU - Taketa, K
AU  - Taketa K
FAU - Watanabe, A
AU  - Watanabe A
FAU - Shimamura, J
AU  - Shimamura J
FAU - Takesue, A
AU  - Takesue A
FAU - Ueda, M
AU  - Ueda M
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Acta Med Okayama
JT  - Acta medica Okayama
JID - 0417611
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.1.43 (Phosphogluconate Dehydrogenase)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.7.- (Phosphotransferases)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.2 (Glucokinase)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
RN  - EC 3.1.3.11 (Fructose-Bisphosphatase)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
RN  - EC 3.1.3.9 (Glucose-6-Phosphatase)
SB  - IM
MH  - Alcohol Oxidoreductases/*metabolism
MH  - Animals
MH  - Cell Differentiation
MH  - *Cell Line
MH  - Epithelial Cells
MH  - Epithelium/enzymology
MH  - Female
MH  - Fructose-Bisphosphatase/metabolism
MH  - Glucokinase/metabolism
MH  - Glucose-6-Phosphatase/metabolism
MH  - Glucosephosphate Dehydrogenase/metabolism
MH  - Hexokinase/metabolism
MH  - Liver/*enzymology
MH  - Male
MH  - Phosphogluconate Dehydrogenase/metabolism
MH  - Phosphoric Monoester Hydrolases/*metabolism
MH  - Phosphotransferases/*metabolism
MH  - Pyruvate Kinase/metabolism
MH  - Rats
EDAT- 1975/04/01 00:00
MHDA- 1975/04/01 00:01
CRDT- 1975/04/01 00:00
PHST- 1975/04/01 00:00 [pubmed]
PHST- 1975/04/01 00:01 [medline]
PHST- 1975/04/01 00:00 [entrez]
PST - ppublish
SO  - Acta Med Okayama. 1975 Apr;29(2):151-9.