PMID- 16968051
OWN - NLM
STAT- MEDLINE
DCOM- 20061102
LR  - 20131121
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 54
IP  - 19
DP  - 2006 Sep 20
TI  - Small molecule microarrays for drug residue detection in foodstuffs.
PG  - 6978-83
AB  - Microarrays have been used as tools for analyzing biological compositions at 
      different levels. In this study, we proposed a small molecule microarray (SMM) 
      method for detection of three veterinary drug residues, chloramphenicol, 
      clenbuterol, and tylosin, in foodstuffs simultaneously and quantitatively. The 
      small drug molecules were immobilized on the surface of the modified glass 
      slides. Then the mixture of drug corresponding antibodies and standards or 
      samples was added to the reaction area. After incubation, the antigen-antibody 
      binding was detected using cy5 labeled secondary antibody. The calibration curves 
      of the residues were drawn, and they indicated the lowest detection limit the 
      linearity range. The detectable concentrations of the three residues are lower 
      than the maximum residue levels (MRLs). No cross reactivity was found among the 
      three residues. The coefficient of variation of the spot intensities was below 5% 
      in a subarray, and below 15% among subarrays. The spike sample test and the 
      comparison of detection results by SMMs and ELISA demonstrated the accuracy of 
      the proposed SMMs method.
FAU - Peng, Zuo
AU  - Peng Z
AD  - State Key Laboratory of Bioreactor Engineering, Department of Food Engineering 
      and Science, East China University of Science and Technology, 130 Meilong Road, 
      Shanghai 200237, China.
FAU - Bang-Ce, Ye
AU  - Bang-Ce Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Veterinary Drugs)
RN  - 66974FR9Q1 (Chloramphenicol)
RN  - XTZ6AXU7KN (Clenbuterol)
RN  - YEF4JXN031 (Tylosin)
SB  - IM
MH  - Chloramphenicol/analysis
MH  - Clenbuterol/analysis
MH  - Drug Residues/*analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Food Analysis/*methods
MH  - *Microarray Analysis
MH  - Sensitivity and Specificity
MH  - Tylosin/analysis
MH  - Veterinary Drugs/analysis
EDAT- 2006/09/14 09:00
MHDA- 2006/11/03 09:00
CRDT- 2006/09/14 09:00
PHST- 2006/09/14 09:00 [pubmed]
PHST- 2006/11/03 09:00 [medline]
PHST- 2006/09/14 09:00 [entrez]
AID - 10.1021/jf061105+ [doi]
PST - ppublish
SO  - J Agric Food Chem. 2006 Sep 20;54(19):6978-83. doi: 10.1021/jf061105+.