PMID- 16968233
OWN - NLM
STAT- MEDLINE
DCOM- 20070126
LR  - 20171116
IS  - 0904-2512 (Print)
IS  - 0904-2512 (Linking)
VI  - 35
IP  - 9
DP  - 2006 Oct
TI  - Matrix metalloproteinase-7, -8, -9, -25, and -26 and CD43, -45, and -68 
      cell-markers in HIV-infected patients' saliva and gingival tissue.
PG  - 530-9
AB  - BACKGROUND: Matrix metalloproteinases (MMPs) process the extracellular matrix and 
      act in tissue remodelling in many physiological and pathological conditions. 
      Certain MMPs can also exert protective anti-inflammatory properties. The levels 
      and expression of MMPs and tissue inhibitors of MMPs (TIMPs) in saliva and 
      gingival tissues of human immunodeficiency virus-seropositive (HIV+) patients are 
      unclear. METHODS: Enzyme-linked immunosorbent assay methods and Western blots 
      were used to study levels and molecular forms of MMP-7, -8, -9, -25, and -26 and 
      TIMP-1 from salivary samples of HIV+ patients (n = 55) and healthy controls (n = 
      10). The expression of MMPs was also studied by immunohistochemical means in 
      gingival tissue specimens (n = 11, HIV+ patients; n = 10, healthy controls). 
      RESULTS: The HIV+ patients' MMP-8 levels in saliva were statistically 
      significantly higher only in the acquired immunodeficiency syndrome (AIDS)-phase. 
      MMP-9 levels in ASX- and AIDS-phases showed increased expression. TIMP-1 levels 
      were significantly decreased in lymphadenopathy syndrome (LAS)- and AIDS-related 
      complex (ARC)-phases, while MMP-8/TIMP-1 and MMP-9/TIMP-1 molar ratios were 
      increased in all phases in comparison with controls. The molecular forms of 
      MMP-7, -25, and -26 were different between patients and controls as assessed by 
      Western blot. Immunohistochemical studies showed slightly enhanced MMP-7, -8, -9, 
      -25, and -26 staining in HIV+ gingival tissue samples in comparison with 
      controls. CONCLUSIONS: This study confirmed and further demonstrated differences 
      in salivary amounts and molecular forms of MMPs and TIMP-1 in HIV+ patients. The 
      results may reflect alterations in host defence reactions associated with HIV 
      infection.
FAU - Mellanen, Liisa
AU  - Mellanen L
AD  - Institute of Dentistry, University of Helsinki, Helsinki, Finland. 
      mellanen@helsinki.fi
FAU - Lahdevirta, Juhani
AU  - Lahdevirta J
FAU - Tervahartiala, Taina
AU  - Tervahartiala T
FAU - Meurman, Jukka H
AU  - Meurman JH
FAU - Sorsa, Timo
AU  - Sorsa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Leukosialin)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Antigens, CD/*analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - Case-Control Studies
MH  - Female
MH  - Gingiva/*chemistry/immunology
MH  - HIV Infections/*immunology
MH  - Horses
MH  - Humans
MH  - Leukocyte Common Antigens/analysis
MH  - Leukosialin/analysis
MH  - Male
MH  - Matrix Metalloproteinases/*analysis
MH  - Mice
MH  - Saliva/*chemistry/immunology
MH  - Tissue Inhibitor of Metalloproteinases/analysis
EDAT- 2006/09/14 09:00
MHDA- 2007/01/27 09:00
CRDT- 2006/09/14 09:00
PHST- 2006/09/14 09:00 [pubmed]
PHST- 2007/01/27 09:00 [medline]
PHST- 2006/09/14 09:00 [entrez]
AID - JOP449 [pii]
AID - 10.1111/j.1600-0714.2006.00449.x [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2006 Oct;35(9):530-9. doi: 10.1111/j.1600-0714.2006.00449.x.