PMID- 16968722
OWN - NLM
STAT- MEDLINE
DCOM- 20070709
LR  - 20061119
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 21
IP  - 12
DP  - 2006 Dec
TI  - Effect of peritoneal dialysis on renal morphology and function.
PG  - 3539-44
AB  - BACKGROUND: Results of clinical studies suggest that peritoneal dialysis (PD) is 
      less harmful to the residual renal function than haemodialysis. However, we have 
      no objective data describing the potential injuring effect of PD to kidney. We 
      studied in rats after unilateral nephrectomy changes in renal structure and 
      function after 12 weeks exposure to standard, glucose-based PD fluid. METHODS: 
      One month after removing one kidney PD catheters were implanted in rats and 
      during the following 12 weeks, twice a day, animals were infused with 20 ml of 
      3.9% glucose dialysis fluid containing high concentration of glucose degradation 
      products. Rats not infused with the dialysis fluid served as control (CON). At 
      the beginning and after 12 weeks of the study renal creatinine clearance, urinary 
      excretion of albumin, N-acetyl-beta-glucosaminidase (NAG) and cytokines were 
      measured. Concentration of malondialdehyde (MDA), advanced glycation end products 
      (AGEs) and monocyte chemoattractant protein-1 (MCP-1) were measured in serum 
      samples. Morphology of the kidneys was evaluated in the light microscope. 
      RESULTS: After 12 weeks exposure to the dialysis fluid serum MDA, AGEs and MCP 
      levels were increased as compared with CON by 80%, P < 0.002, 29%, P < 0.05 and 
      71%, P < 0.005, respectively. Renal clearance of creatinine was comparable in 
      both groups, but urinary excretion of albumin was increased by 55% in control 
      group and by 160% in the studied group, P < 0.001; whereas urinary excretion of 
      NAG was not changed in control group but increased by 125% in the studied group, 
      P < 0.01. Increase of the remnant kidney's weight was higher (+77%, P < 0.01) in 
      the CON group, but accumulation of the extramesangial matrix in glomeruli and 
      collagen in the peritubular space was stronger in the studied group by 69%, P < 
      0.0001 and 274%, P < 0.0001, respectively. CONCLUSION: Chronic exposure of rats 
      to the glucose-based dialysis fluid causes morphological changes in the renal 
      glomeruli similar to diabetic nephropathy. Albuminuria increases what may 
      accelerate progression of the kidney damage.
FAU - Breborowicz, Andrzej
AU  - Breborowicz A
AD  - Department of Pathophysiology, Poznan Medical School, Ul. Swiecickiego 6, 60-781 
      Poznan, Poland. abreb@am.poznan.pl
FAU - Pawlaczyk, Krzysztof
AU  - Pawlaczyk K
FAU - Polubinska, Alicja
AU  - Polubinska A
FAU - Gorna, Kinga
AU  - Gorna K
FAU - Wieslander, Anders
AU  - Wieslander A
FAU - Carlsson, Ola
AU  - Carlsson O
FAU - Tam, Paul
AU  - Tam P
FAU - Wu, George
AU  - Wu G
LA  - eng
PT  - Journal Article
DEP - 20060912
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
SB  - IM
MH  - Animals
MH  - Kidney/*pathology/*physiopathology
MH  - Male
MH  - Peritoneal Dialysis/*adverse effects
MH  - Rats
MH  - Rats, Wistar
EDAT- 2006/09/14 09:00
MHDA- 2007/07/10 09:00
CRDT- 2006/09/14 09:00
PHST- 2006/09/14 09:00 [pubmed]
PHST- 2007/07/10 09:00 [medline]
PHST- 2006/09/14 09:00 [entrez]
AID - gfl402 [pii]
AID - 10.1093/ndt/gfl402 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2006 Dec;21(12):3539-44. doi: 10.1093/ndt/gfl402. Epub 
      2006 Sep 12.