PMID- 16968896
OWN - NLM
STAT- MEDLINE
DCOM- 20070830
LR  - 20220409
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 109
IP  - 2
DP  - 2007 Jan 15
TI  - Differentiation of CD1a- and CD1a+ monocyte-derived dendritic cells is biased by 
      lipid environment and PPARgamma.
PG  - 643-52
AB  - Accumulating data have shown that the microenvironment of dendritic cells 
      modulates subtype differentiation and CD1 expression, but the mechanisms by which 
      exogenous factors confer these effects are poorly understood. Here we describe 
      the dependence of CD1a- monocyte-derived dendritic cell (moDC) development on 
      lipids associated with the expression of peroxisome proliferator-activated 
      receptor-gamma (PPARgamma). We also show the consecutive differentiation of 
      immature CD1a-PPARgamma+ moDCs to CD1a+PPARgamma- cells limited by serum 
      lipoproteins and terminated by proinflammatory cytokines. Immature CD1a- moDCs 
      possess higher internalizing capacity than CD1a+ cells, whereas both activated 
      subtypes have similar migratory potential but differ in their cytokine and 
      chemokine profiles, which translates to distinct T-lymphocyte-polarizing 
      capacities. CD1a+ moDCs stand out by their capability to secrete high amounts of 
      IL-12p70 and CCL1. As lipoproteins skew moDC differentiation toward the 
      generation of CD1a-PPARgamma+ cells and inhibit the development of 
      CD1a+PPARgamma- cells, we suggest that the uptake of lipids results in endogenous 
      PPARgamma agonists that induce a cascade of gene transcription coordinating lipid 
      metabolism, the expression of lipid-presenting CD1 molecules, subtype dichotomy, 
      and function. The presence of CD1a-PPARgamma+ and CD1a+PPARgamma- DCs in lymph 
      nodes and in pulmonary Langerhans cell histiocytosis confirms the functional 
      relevance of these DC subsets in vivo.
FAU - Gogolak, Peter
AU  - Gogolak P
AD  - Institute of Immunology, Department of Pathology, Research Center for Molecular 
      Medicine, University of Debrecen, 98 Nagyerdei Blv Debrecen, H-4012 Hungary.
FAU - Rethi, Bence
AU  - Rethi B
FAU - Szatmari, Istvan
AU  - Szatmari I
FAU - Lanyi, Arpad
AU  - Lanyi A
FAU - Dezso, Balazs
AU  - Dezso B
FAU - Nagy, Laszlo
AU  - Nagy L
FAU - Rajnavolgyi, Eva
AU  - Rajnavolgyi E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060912
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1a antigen)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Lipids)
RN  - 0 (PPAR gamma)
SB  - IM
MH  - Antigens, CD1/*biosynthesis
MH  - Cell Differentiation/*immunology
MH  - Chemokines/biosynthesis
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/cytology/*immunology
MH  - Humans
MH  - Lipids/*physiology
MH  - Monocytes/cytology/*immunology
MH  - PPAR gamma/*physiology
MH  - Phenotype
EDAT- 2006/09/14 09:00
MHDA- 2007/08/31 09:00
CRDT- 2006/09/14 09:00
PHST- 2006/09/14 09:00 [pubmed]
PHST- 2007/08/31 09:00 [medline]
PHST- 2006/09/14 09:00 [entrez]
AID - S0006-4971(20)52075-2 [pii]
AID - 10.1182/blood-2006-04-016840 [doi]
PST - ppublish
SO  - Blood. 2007 Jan 15;109(2):643-52. doi: 10.1182/blood-2006-04-016840. Epub 2006 
      Sep 12.