PMID- 16969008
OWN - NLM
STAT- MEDLINE
DCOM- 20070130
LR  - 20191210
IS  - 1015-3837 (Print)
IS  - 1015-3837 (Linking)
VI  - 21
IP  - 6
DP  - 2006
TI  - Automated microscopy of amniotic fluid cells: detection of FISH signals using the 
      FastFISH imaging system.
PG  - 523-7
AB  - OBJECTIVE: FISH (fluorescence in situ hybridization) analysis is a valuable 
      adjunct to cytogenetics that provides a rapid screen for common abnormalities. 
      However, FISH is expensive, labor-intensive, and requires a high skill level and 
      subjective signal interpretation. A fully automated system for FISH analysis 
      could improve laboratory efficiency and potentially reduce errors and costs. 
      METHODS: In this study we blindly compared automated FISH signal acquisition and 
      display against standard FISH analysis. A total of 62 amniocentesis samples were 
      prepared using the AneuVysion multicolor DNA probe kit and probed for chromosomes 
      13, 18, 21, X, and Y. Two sets of slides were produced from each sample. Fifty 
      cells were scored in each slide. One set was evaluated using standard manual 
      microscopy and the other using the automated image acquisition and display 
      capabilities of the Ikoniscope fastFISH amnio Test System. This system uses 
      epifluorescence optics, along with optimized slide management to process slides 
      automatically. RESULTS: A 100% concordance was observed between the results 
      obtained using manual microscopy and the automated system. There was also 100% 
      concordance between the FISH results and those obtained by conventional 
      karyotyping. CONCLUSION: Our data suggest that the automated system is capable of 
      providing accurate and rapid identification and display of cells and FISH 
      signals.
FAU - Evans, Mark I
AU  - Evans MI
AD  - Comprehensive Genetics, New York, NY 10021, USA. Evans@CompreGen.com
FAU - Sharp, Michael
AU  - Sharp M
FAU - Tepperberg, James
AU  - Tepperberg J
FAU - Kilpatrick, Michael W
AU  - Kilpatrick MW
FAU - Tsipouras, Petros
AU  - Tsipouras P
FAU - Tafas, Triantafyllos
AU  - Tafas T
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20060912
PL  - Switzerland
TA  - Fetal Diagn Ther
JT  - Fetal diagnosis and therapy
JID - 9107463
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Amniocentesis
MH  - Chromosome Disorders/*diagnosis
MH  - Female
MH  - Humans
MH  - Image Processing, Computer-Assisted/instrumentation
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/methods
MH  - Microscopy, Fluorescence/instrumentation
MH  - Middle Aged
MH  - Pregnancy
MH  - Sensitivity and Specificity
EDAT- 2006/09/14 09:00
MHDA- 2007/01/31 09:00
CRDT- 2006/09/14 09:00
PHST- 2006/01/05 00:00 [received]
PHST- 2006/01/05 00:00 [accepted]
PHST- 2006/09/14 09:00 [pubmed]
PHST- 2007/01/31 09:00 [medline]
PHST- 2006/09/14 09:00 [entrez]
AID - 95666 [pii]
AID - 10.1159/000095666 [doi]
PST - ppublish
SO  - Fetal Diagn Ther. 2006;21(6):523-7. doi: 10.1159/000095666. Epub 2006 Sep 12.