PMID- 16969294
OWN - NLM
STAT- MEDLINE
DCOM- 20061025
LR  - 20061115
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 82
IP  - 5
DP  - 2006 Sep 15
TI  - Critical roles of memory T cells and antidonor immunoglobulin in rejection of 
      allogeneic bone marrow cells in sensitized recipient mice.
PG  - 689-98
AB  - BACKGROUND: Allosensitization is a major risk factor for graft failure in 
      clinical bone marrow transplantation, even with an human leukocyte antigen 
      (HLA)-matched combination under radiation-based conditioning regimens. The 
      critical components of immunological memory in donor bone marrow graft rejection 
      in allosensitized hosts remain unclear at present. METHODS: C57BL/6-recipient 
      mice, which had been intraperitoneally injected with splenocytes from donor C3H 
      mice on day -35 (sensitized recipients), had been lethally irradiated with 10-Gy 
      whole-body irradiation and were intravenously injected with T-cell-depleted bone 
      marrow cells (TCD-BMC) from C3H mice or third-party SJL mice. RESULTS: Lethally 
      irradiated recipient mice, which had been sensitized by donor splenocytes 5 weeks 
      before the transplantation of TCD-BMC, completely rejected the donor-BMC in a 
      donor-specific manner, whereas none of the nonsensitized recipient mice, all of 
      which showed full allogeneic chimerism, rejected the donor TCD-BMC. 
      Antibody-mediated T cell and/or Natural Killer (NK) cell depletion did not 
      improve the ability of the sensitized recipients to overcome the rejection even 
      when a megadose of TCD-BMC was administered to the sensitized recipients. 
      Furthermore, BMC rejection occurred in sensitized B cell-deficient mice. In 
      adoptive transfer experiments, naive mice, which received a transfer of purified 
      T cells from sensitized mice, rejected the donor BMC, but not those from 
      nonsensitized mice. Moreover, naive mice, which received a transfer of serum 
      containing antidonor immunoglobulin, rejected the donor BMC. CONCLUSIONS: These 
      findings suggest that alloreactive memory T cells and antidonor immunoglobulin 
      independently function to reject donor BMC in sensitized recipients.
FAU - Nagata, Shigeyuki
AU  - Nagata S
AD  - Division of Pathophygiological and Experimental Pathology, Department of 
      Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 
      Japan.
FAU - Okano, Shinji
AU  - Okano S
FAU - Yonemitsu, Yoshikazu
AU  - Yonemitsu Y
FAU - Nakagawa, Kazunori
AU  - Nakagawa K
FAU - Tomita, Yukihiro
AU  - Tomita Y
FAU - Yoshikai, Yasunobu
AU  - Yoshikai Y
FAU - Shimada, Mitsuo
AU  - Shimada M
FAU - Maehara, Yoshihiko
AU  - Maehara Y
FAU - Sueishi, Katsuo
AU  - Sueishi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Bone Marrow Transplantation/*immunology
MH  - Graft Rejection/*immunology
MH  - Immunization/*methods
MH  - *Immunologic Memory
MH  - Lymphocyte Transfusion
MH  - Mice
MH  - Models, Animal
MH  - T-Lymphocytes/*immunology/transplantation
MH  - Tissue Donors
MH  - Transplantation, Homologous/immunology
EDAT- 2006/09/14 09:00
MHDA- 2006/10/26 09:00
CRDT- 2006/09/14 09:00
PHST- 2006/09/14 09:00 [pubmed]
PHST- 2006/10/26 09:00 [medline]
PHST- 2006/09/14 09:00 [entrez]
AID - 00007890-200609150-00015 [pii]
AID - 10.1097/01.tp.0000235589.66683.0e [doi]
PST - ppublish
SO  - Transplantation. 2006 Sep 15;82(5):689-98. doi: 
      10.1097/01.tp.0000235589.66683.0e.