PMID- 16971421
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 80
IP  - 23
DP  - 2006 Dec
TI  - A full-length cDNA infectious clone of North American type 1 porcine reproductive 
      and respiratory syndrome virus: expression of green fluorescent protein in the 
      Nsp2 region.
PG  - 11447-55
AB  - The recent emergence of a unique group of North American type 1 porcine 
      reproductive and respiratory syndrome virus (PRRSV) in the United States presents 
      new disease control problems for a swine industry that has already been impacted 
      seriously by North American type 2 PRRSV. In this study, a full-length cDNA 
      infectious clone was generated from a low-virulence North American type 1 PRRSV 
      isolate, SD01-08. In vitro studies demonstrated that the cloned virus maintained 
      growth properties similar to those of the parental virus. Virological, 
      pathological, and immunological observations from animals challenged with cloned 
      viruses were similar to those from animals challenged with the parental virus and 
      a modified live virus vaccine. To further explore the potential use as a viral 
      backbone for expressing foreign genes, the green fluorescent protein (GFP) was 
      inserted into a unique deletion site located at amino acid positions 348 and 349 
      of the predicted Nsp2 region in the virus, and expression of the Nsp2-GFP fusion 
      protein was visualized by fluorescent microscopy. The availability of this North 
      American type 1 infectious clone provides an important research tool for further 
      study of the basic viral biology and pathogenic mechanisms of this group of type 
      1 PRRSV in the United States.
FAU - Fang, Ying
AU  - Fang Y
AD  - Center for Infectious Disease Research and Vaccinology, Department of Veterinary 
      Science, Box 2175, South Dakota State University, Brookings, SD 57007-1396, USA. 
      ying.fang@sdstate.edu
FAU - Rowland, Raymond R R
AU  - Rowland RR
FAU - Roof, Michael
AU  - Roof M
FAU - Lunney, Joan K
AU  - Lunney JK
FAU - Christopher-Hennings, Jane
AU  - Christopher-Hennings J
FAU - Nelson, Eric A
AU  - Nelson EA
LA  - eng
SI  - GENBANK/DQ489311
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20060913
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Viral Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary/analysis/*physiology
MH  - DNA, Viral/biosynthesis
MH  - Gene Expression Regulation, Viral
MH  - Green Fluorescent Proteins/metabolism
MH  - Molecular Sequence Data
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/growth & 
      development/isolation & purification/*pathogenicity
MH  - Swine
MH  - Viral Nonstructural Proteins/chemistry/*genetics
MH  - Viral Proteins/chemistry/genetics
PMC - PMC1642622
EDAT- 2006/09/15 09:00
MHDA- 2007/01/17 09:00
PMCR- 2007/04/01
CRDT- 2006/09/15 09:00
PHST- 2006/09/15 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/09/15 09:00 [entrez]
PHST- 2007/04/01 00:00 [pmc-release]
AID - JVI.01032-06 [pii]
AID - 1032-06 [pii]
AID - 10.1128/JVI.01032-06 [doi]
PST - ppublish
SO  - J Virol. 2006 Dec;80(23):11447-55. doi: 10.1128/JVI.01032-06. Epub 2006 Sep 13.