PMID- 16973200
OWN - NLM
STAT- MEDLINE
DCOM- 20070112
LR  - 20220316
IS  - 0041-0101 (Print)
IS  - 0041-0101 (Linking)
VI  - 48
IP  - 6
DP  - 2006 Nov
TI  - Species selective resistance of cardiac muscle voltage gated sodium channels: 
      characterization of brevetoxin and ciguatoxin binding sites in rats and fish.
PG  - 702-12
AB  - Brevetoxins (PbTxs) and ciguatoxins (CTXs) are two suites of dinoflagellate 
      derived marine polyether neurotoxins that target the voltage gated sodium channel 
      (VGSC). PbTxs are commonly responsible for massive fish kills and unusual 
      mortalities in marine mammals. CTXs, more often noted for human intoxication, are 
      suspected causes of fish and marine mammal intoxication, although this has never 
      been reported in the field. VGSCs, present in the membrane of all excitable cells 
      including those found in skeletal muscle, nervous and heart tissues, are found as 
      isoforms with differential expression within species and tissues. To investigate 
      the tissue and species susceptibility to these biotoxins, we determined the 
      relative affinity of PbTx-2 and -3 and P-CTX-1 to native VGSCs in the brain, 
      heart, and skeletal muscle of rat and the marine teleost fish Centropristis 
      striata by competitive binding in the presence of [(3)H]PbTx-3. No differences 
      between rat and fish were observed in the binding of PbTxs and CTX to either 
      brain or skeletal muscle. However, [(3)H]PbTx-3 showed substantial lower affinity 
      to rat heart tissue while in the fish it bound with the same affinity to heart 
      than to brain or skeletal muscle. These new insights into PbTxs and CTXs binding 
      in fish and mammalian excitable tissues indicate a species related resistance of 
      heart VGSC in the rat; yet, with comparable sensitivity between the species for 
      brain and skeletal muscle.
FAU - Dechraoui, Marie-Yasmine Bottein
AU  - Dechraoui MY
AD  - Marine Biotoxins Program, Center for Coastal Environmental Health and 
      Biomolecular Research; NOAA-National Ocean Service, Charleston, SC 29412, USA.
FAU - Wacksman, Jeremy J
AU  - Wacksman JJ
FAU - Ramsdell, John S
AU  - Ramsdell JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060812
PL  - England
TA  - Toxicon
JT  - Toxicon : official journal of the International Society on Toxinology
JID - 1307333
RN  - 0 (Fish Proteins)
RN  - 0 (Marine Toxins)
RN  - 0 (Neurotoxins)
RN  - 0 (Oxocins)
RN  - 0 (Sodium Channels)
RN  - 11050-21-8 (Ciguatoxins)
RN  - 98225-48-0 (brevetoxin)
SB  - IM
MH  - Animals
MH  - Bass/*metabolism
MH  - Brain/cytology/metabolism
MH  - Ciguatoxins/chemistry/metabolism/*pharmacology
MH  - Dinoflagellida/metabolism
MH  - Fish Proteins/chemistry/*drug effects/metabolism
MH  - Marine Toxins/chemistry/metabolism/*pharmacology
MH  - Muscle Fibers, Skeletal/metabolism
MH  - Myocytes, Cardiac/chemistry/*drug effects/metabolism
MH  - Neurotoxins/chemistry/metabolism/*pharmacology
MH  - Oxocins/chemistry/metabolism/*pharmacology
MH  - Rats
MH  - Sodium Channels/chemistry/*drug effects/metabolism
MH  - Species Specificity
EDAT- 2006/09/16 09:00
MHDA- 2007/01/16 09:00
CRDT- 2006/09/16 09:00
PHST- 2006/04/14 00:00 [received]
PHST- 2006/07/26 00:00 [revised]
PHST- 2006/07/28 00:00 [accepted]
PHST- 2006/09/16 09:00 [pubmed]
PHST- 2007/01/16 09:00 [medline]
PHST- 2006/09/16 09:00 [entrez]
AID - S0041-0101(06)00291-1 [pii]
AID - 10.1016/j.toxicon.2006.07.032 [doi]
PST - ppublish
SO  - Toxicon. 2006 Nov;48(6):702-12. doi: 10.1016/j.toxicon.2006.07.032. Epub 2006 Aug 
      12.