PMID- 16973234 OWN - NLM STAT- MEDLINE DCOM- 20070322 LR - 20121115 IS - 0168-1656 (Print) IS - 0168-1656 (Linking) VI - 127 IP - 4 DP - 2007 Jan 20 TI - Biodegradation kinetics of 2,4-dichlorophenol by acclimated mixed cultures. PG - 716-26 AB - The biodegradation kinetics of 2,4-dichlorophenol (2,4-DCP) by culture (Culture M) acclimated to mixture of 4-chlorophenol (4-CP) and 2,4-DCP and the culture (Culture 4) acclimated to 4-CP only were investigated in aerobic batch reactors. Also, pure strains isolated from mixed cultures were searched for their ability towards the biodegradation of 2,4-DCP. Culture 4 was able to completely degrade 2,4-DCP up to 80 mg/L within 30 h and removal efficiency dropped to 21% upon increasing initial concentration to 108.8 mg/L. When the Culture M was used, complete degradation of 2,4-DCP in the range of 12.5-104.4 mg/L was attained. A linear relationship between time required for complete degradation and initial 2,4-DCP concentrations was observed for both mixed cultures. It was observed that the Haldane equation can be used to predict specific degradation rate (SDR) (R(2)>0.99) as a function of initial 2,4-DCP concentrations and it adequately describes 2,4-DCP concentration profiles. Both of the mixed cultures settled well, which is important to maintain good removal efficiency for longer periods of time for real full-scale applications. Although the pure strains isolated from mixed cultures were found to have higher SDR of 2,4-DCP compared to mixed cultures, they did not settle well under quiescent conditions. FAU - Sahinkaya, Erkan AU - Sahinkaya E AD - Middle East Technical University, Environmental Engineering Department, 06531 Ankara, Turkey. erkans@metu.edu.tr FAU - Dilek, Filiz B AU - Dilek FB LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20060914 PL - Netherlands TA - J Biotechnol JT - Journal of biotechnology JID - 8411927 RN - 0 (Chlorophenols) RN - 0 (Culture Media) RN - 3DLC36A01X (4-chlorophenol) RN - R669TG1950 (2,4-dichlorophenol) SB - IM MH - Bacteria/*metabolism MH - Biodegradation, Environmental MH - *Bioreactors MH - Cells, Cultured MH - Chlorophenols/*chemistry/*metabolism MH - Culture Media MH - Kinetics MH - Time Factors EDAT- 2006/09/16 09:00 MHDA- 2007/03/23 09:00 CRDT- 2006/09/16 09:00 PHST- 2006/03/21 00:00 [received] PHST- 2006/06/26 00:00 [revised] PHST- 2006/08/05 00:00 [accepted] PHST- 2006/09/16 09:00 [pubmed] PHST- 2007/03/23 09:00 [medline] PHST- 2006/09/16 09:00 [entrez] AID - S0168-1656(06)00698-5 [pii] AID - 10.1016/j.jbiotec.2006.08.009 [doi] PST - ppublish SO - J Biotechnol. 2007 Jan 20;127(4):716-26. doi: 10.1016/j.jbiotec.2006.08.009. Epub 2006 Sep 14.