PMID- 16973544
OWN - NLM
STAT- MEDLINE
DCOM- 20061024
LR  - 20191210
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 80
IP  - 19
DP  - 2006 Oct
TI  - ICP22 is required for wild-type composition and infectivity of herpes simplex 
      virus type 1 virions.
PG  - 9381-90
AB  - The immediate-early regulatory protein ICP22 is required for efficient 
      replication of herpes simplex virus type 1 in some cell types (permissive) but 
      not in others (restrictive). In mice infected via the ocular route, the 
      pathogenesis of an ICP22- virus, 22/n199, was altered relative to that of 
      wild-type virus. Specifically, tear film titers of 22/n199-infected mice were 
      significantly reduced at 3 h postinfection relative to those of mice infected 
      with wild-type virus. Further, 22/n199 virus titers were below the level of 
      detection in trigeminal ganglia (TG) during the first 9 days postinfection. On 
      day 30 postinfection, TG from 22/n199-infected mice contained reduced viral 
      genome loads and exhibited reduced expression of latency-associated transcripts 
      and reduced reactivation efficiency relative to TG from wild-type virus-infected 
      mice. Notably, the first detectable alteration in the pathogenesis of 22/n199 in 
      these tests occurred in the eye prior to the onset of nascent virus production. 
      Thus, ICP22- virions appeared to be degraded, cleared, or adsorbed more rapidly 
      than wild-type virions, implying potential differences in the composition of the 
      two virion types. Analysis of the protein composition of purified extracellular 
      virions indicated that ICP22 is not a virion component and that 22/n199 virions 
      sediment at a reduced density relative to wild-type virions. Although similar to 
      wild-type virions morphologically, 22/n199 virions contain reduced amounts of two 
      gamma2 late proteins, US11 and gC, and increased amounts of two immediate-early 
      proteins, ICP0 and ICP4, as well as protein species not detected in wild-type 
      virions. Although ICP22- viruses replicate to near-wild-type levels in permissive 
      cells, the virions produced in these cells are biochemically and physically 
      different from wild-type virions. These virion-specific differences in ICP22- 
      viruses add a new level of complexity to the functional analysis of this 
      immediate-early viral regulatory protein.
FAU - Orlando, Joseph S
AU  - Orlando JS
AD  - Department of Microbiology and Molecular Genetics, Harvard Medical School at the 
      Beth Israel Deaconess Medical Center, 330 Brookline Avenue, RN 123, Boston, MA 
      02215, USA.
FAU - Balliet, John W
AU  - Balliet JW
FAU - Kushnir, Anna S
AU  - Kushnir AS
FAU - Astor, Todd L
AU  - Astor TL
FAU - Kosz-Vnenchak, Magdalena
AU  - Kosz-Vnenchak M
FAU - Rice, Stephen A
AU  - Rice SA
FAU - Knipe, David M
AU  - Knipe DM
FAU - Schaffer, Priscilla A
AU  - Schaffer PA
LA  - eng
GR  - R01 CA20260/CA/NCI NIH HHS/United States
GR  - F32 CA99887/CA/NCI NIH HHS/United States
GR  - R01 CA020260/CA/NCI NIH HHS/United States
GR  - F32 CA099887/CA/NCI NIH HHS/United States
GR  - R01 AI50127/AI/NIAID NIH HHS/United States
GR  - R01 AI050127/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (ICP22 protein, human herpesvirus 1)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Viral Regulatory and Accessory Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Genome, Viral/genetics
MH  - Herpes Simplex/genetics/pathology/*virology
MH  - Herpesvirus 1, Human/*chemistry/*physiology
MH  - Humans
MH  - Immediate-Early Proteins/isolation & purification/*metabolism
MH  - Kinetics
MH  - Male
MH  - Mice
MH  - Mutation/genetics
MH  - Trigeminal Ganglion/metabolism/pathology/virology
MH  - Viral Regulatory and Accessory Proteins
MH  - Virion/*chemistry/genetics/isolation & purification/*physiology
PMC - PMC1617265
EDAT- 2006/09/16 09:00
MHDA- 2006/10/25 09:00
PMCR- 2007/02/01
CRDT- 2006/09/16 09:00
PHST- 2006/09/16 09:00 [pubmed]
PHST- 2006/10/25 09:00 [medline]
PHST- 2006/09/16 09:00 [entrez]
PHST- 2007/02/01 00:00 [pmc-release]
AID - 80/19/9381 [pii]
AID - 1061-06 [pii]
AID - 10.1128/JVI.01061-06 [doi]
PST - ppublish
SO  - J Virol. 2006 Oct;80(19):9381-90. doi: 10.1128/JVI.01061-06.