PMID- 16973547
OWN - NLM
STAT- MEDLINE
DCOM- 20061024
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 80
IP  - 19
DP  - 2006 Oct
TI  - Tissue-specific splicing of the herpes simplex virus type 1 latency-associated 
      transcript (LAT) intron in LAT transgenic mice.
PG  - 9414-23
AB  - To study the regulation of herpes simplex virus type 1 (HSV-1) latency-associated 
      transcript (LAT) expression and processing in the absence of other cis and trans 
      viral functions, a transgenic mouse containing the region encompassing the LAT 
      promoter (LAP1) and the LAT 5' exon through the 2.0-kb intron was created. LAT 
      expression was detectable by reverse transcriptase PCR (RT-PCR) in a number of 
      tissues, including the dorsal root ganglia (DRG), trigeminal ganglia (TG), brain, 
      skin, liver, and kidney. However, when the accumulation of the 2.0-kb LAT intron 
      was analyzed at the cellular level by in situ hybridization, little or no 
      detectable accumulation was observed in the brain, spinal cord, kidney, or foot, 
      although the 2.0-kb LAT intron was detected at high levels (over 90% of neurons) 
      in the DRG and TG. Northern blot analysis detected the stable 2.0-kb LAT intron 
      only in the sensory ganglia. When relative amounts of the spliced and unspliced 
      LAT within the brain, liver, kidney, spinal cord, TG, and DRG were analyzed by 
      real-time RT-PCR, splicing of the 2.0-kb LAT intron was significantly more 
      efficient in the sensory ganglia than in other tissues. Finally, infection of 
      both transgenic mice and nontransgenic littermates with HSV-1 revealed no 
      differences in lytic replication, establishment of latency, or reactivation, 
      suggesting that expression of the LAT transgene in trans has no significant 
      effect on those functions. Taken together, these data indicate that the 
      regulation of expression and processing of LAT RNA within the mouse is highly 
      cell-type specific and occurs in the absence of other viral cis- and trans-acting 
      factors.
FAU - Gussow, Anne M
AU  - Gussow AM
AD  - Department of Molecular Genetics and Microbiology, Box 100266, University of 
      Florida College of Medicine, Gainesville, FL 32610-0266, USA.
FAU - Giordani, Nicole V
AU  - Giordani NV
FAU - Tran, Robert K
AU  - Tran RK
FAU - Imai, Yumi
AU  - Imai Y
FAU - Kwiatkowski, Dacia L
AU  - Kwiatkowski DL
FAU - Rall, Glenn F
AU  - Rall GF
FAU - Margolis, Todd P
AU  - Margolis TP
FAU - Bloom, David C
AU  - Bloom DC
LA  - eng
GR  - EY10008/EY/NEI NIH HHS/United States
GR  - R01 NS040500/NS/NINDS NIH HHS/United States
GR  - NS40500/NS/NINDS NIH HHS/United States
GR  - R01 AI048633/AI/NIAID NIH HHS/United States
GR  - R01 EY010008/EY/NEI NIH HHS/United States
GR  - EY02162/EY/NEI NIH HHS/United States
GR  - AI07110/AI/NIAID NIH HHS/United States
GR  - AI48633/AI/NIAID NIH HHS/United States
GR  - T32 AI007110/AI/NIAID NIH HHS/United States
GR  - P30 EY002162/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
SB  - IM
MH  - Acute Disease
MH  - Aging/physiology
MH  - Animals
MH  - Exons/genetics
MH  - Ganglia, Sensory/pathology/virology
MH  - Herpes Simplex/*pathology/*virology
MH  - Herpesvirus 1, Human/*genetics/*physiology
MH  - Introns/*genetics
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Transgenic
MH  - Neurons/pathology/virology
MH  - Organ Specificity
MH  - Promoter Regions, Genetic/genetics
MH  - RNA Splicing/*genetics
MH  - Transcription, Genetic/genetics
MH  - Virus Latency/*genetics
PMC - PMC1617271
EDAT- 2006/09/16 09:00
MHDA- 2006/10/25 09:00
PMCR- 2007/02/01
CRDT- 2006/09/16 09:00
PHST- 2006/09/16 09:00 [pubmed]
PHST- 2006/10/25 09:00 [medline]
PHST- 2006/09/16 09:00 [entrez]
PHST- 2007/02/01 00:00 [pmc-release]
AID - 80/19/9414 [pii]
AID - 0530-06 [pii]
AID - 10.1128/JVI.00530-06 [doi]
PST - ppublish
SO  - J Virol. 2006 Oct;80(19):9414-23. doi: 10.1128/JVI.00530-06.