PMID- 1697610
OWN - NLM
STAT- MEDLINE
DCOM- 19901011
LR  - 20190827
IS  - 0021-9975 (Print)
IS  - 0021-9975 (Linking)
VI  - 103
IP  - 1
DP  - 1990 Jul
TI  - An immunohistochemical study of the topography and cellular localization of three 
      neural proteins in the sheep nervous system.
PG  - 23-35
AB  - The peroxidase-anti-peroxidase (PAP) method was used to determine the topography 
      and cellular localization of glial fibrillary acidic protein (GFAP), myelin basic 
      protein (MBP) and carbonic anhydrase II (CAII) in the central nervous system 
      (CNS), dorsal root ganglia and dorsal and ventral spinal nerve roots of the 
      sheep. Parallel studies of mouse brain provided comparative data. Several 
      fixatives were compared for their relative merits in preserving marker protein 
      expression: GFAP was well preserved irrespective of the fixative employed; MBP 
      was best preserved in formal sublimate and CAII was best preserved in Carnoy's 
      fluid. In sheep, GFAP expression was seen in protoplasmic and fibrous astrocytes, 
      Bergmann glial cells, a proportion of ependymal cells, amphicytes of spinal 
      ganglia and in a proportion of presumed Schwann cells of dorsal and ventral 
      spinal nerve roots. MBP expression was seen in mature and developing myelin 
      sheaths of the central nervous system and in the cytoplasm of sparse myelinating 
      oligodendroglia of the sub-cortical white matter of the cerebrum. CAII expression 
      was seen in choroid plexus epithelium in all ages of sheep studied and, in a 
      young lamb and an adult sheep, in glia and neuropil of ventral horn grey matter 
      of the spinal cord and in the cytoplasm of white matter glia, presumed fibrous 
      astrocytes, throughout the CNS. Compared with sheep brain, mouse brain showed the 
      following differences in marker protein localization. GFAP was weakly expressed 
      by protoplasmic astrocytes and not expressed in ependyma, oligodendroglia 
      expressing intracytoplasmic MBP were frequent and widespread in neonatal mouse 
      brain, CAII was expressed in myelin and oligodendroglia.(ABSTRACT TRUNCATED AT 
      250 WORDS)
FAU - Jeffrey, M
AU  - Jeffrey M
AD  - Lasswade Veterinary Laboratory, Penicuik, Midlothian, Scotland, U.K.
FAU - Wells, G A
AU  - Wells GA
FAU - Bridges, A W
AU  - Bridges AW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Comp Pathol
JT  - Journal of comparative pathology
JID - 0102444
RN  - 0 (Biomarkers)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Myelin Basic Protein)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Animals, Newborn
MH  - Biomarkers
MH  - *Brain Chemistry
MH  - Carbonic Anhydrases/*analysis
MH  - Ganglia, Spinal/analysis/enzymology
MH  - Glial Fibrillary Acidic Protein/*analysis
MH  - Immunoenzyme Techniques/veterinary
MH  - Mice
MH  - Myelin Basic Protein/*analysis
MH  - Myelin Sheath/analysis/enzymology
MH  - Sheep/*anatomy & histology
MH  - Spinal Cord/analysis/*enzymology
EDAT- 1990/07/01 00:00
MHDA- 1990/07/01 00:01
CRDT- 1990/07/01 00:00
PHST- 1990/07/01 00:00 [pubmed]
PHST- 1990/07/01 00:01 [medline]
PHST- 1990/07/01 00:00 [entrez]
AID - S0021-9975(08)80132-6 [pii]
AID - 10.1016/s0021-9975(08)80132-6 [doi]
PST - ppublish
SO  - J Comp Pathol. 1990 Jul;103(1):23-35. doi: 10.1016/s0021-9975(08)80132-6.