PMID- 16978709
OWN - NLM
STAT- MEDLINE
DCOM- 20070302
LR  - 20181201
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 114
IP  - 3-4
DP  - 2006 Dec 15
TI  - Characterization of conventional and plasmacytoid dendritic cells in swine 
      secondary lymphoid organs and blood.
PG  - 224-37
AB  - Dendritic cells (DCs) act as antigen presenting cells that bridge innate and 
      adaptive immune systems with the unique capacity to initiate primary T-cell 
      responses and efficiently stimulate memory responses. In pig, little information 
      is available about these cells in secondary lymphoid organs, the place where T 
      cell activation usually occurs. As increased knowledge on DC is a necessary 
      prerequisite to further understand their role in response to microbial infection 
      or in protection after vaccination, we investigated the DC types that would be 
      present in tonsil, spleen and non-subcutaneous lymph nodes in the steady state. 
      One population was composed of CD172a(+)CD11R1(+)CD1(+/-)CD80/86(+/-) cells and 
      would correspond to conventional DCs (cDC), while the other one was composed of 
      CD172a(+)CD4(+)CD1(+/-)CD80/86(+/-) cells and would correspond to plasmacytoid 
      DCs (pDC). These subsets were also detected in blood but spleen was the tissue 
      with the higher frequency of such DCs. In lymphoid organs, most of cDC and pDC 
      were in an immature status, as revealed by the low percentage of cells expressing 
      the co-stimulatory molecule CD80/86. However, expression of that marker by 5% of 
      DCs in organs and up to 15% in blood, together with lower expression of CD1a and 
      expression of CD208, would indicate a partial activation and/or semi-maturation. 
      Interestingly, 8% of tonsil pDC and 15% of blood pDC were shown to secrete 
      IFN-alpha, while 18-20% of cDC expressed TNF-alpha in these tissues. Both cell 
      types also expressed IL-12 and IL-10 in the steady state. Measurements of 
      IFN-alpha, TNF-alpha, IL-12 and IL-10 levels in serum confirmed their production 
      within immune homeostasis, whereas IL-6, IL-18 and IFN-gamma could not be 
      detected. Altogether, these data complete knowledge on porcine immune system 
      cells and will be a useful tool for further in vivo studies on porcine DC role in 
      peripheral tolerance induction and in immune responses to pathogens.
FAU - Jamin, Agnes
AU  - Jamin A
AD  - French Agency for Food Safety (AFSSA), Poultry and Swine Research Laboratory, 
      Swine Virology Immunology Unit, Ploufragan, France.
FAU - Gorin, Stephane
AU  - Gorin S
FAU - Le Potier, Marie-Frederique
AU  - Le Potier MF
FAU - Kuntz-Simon, Gaelle
AU  - Kuntz-Simon G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060914
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Animals
MH  - Antigens, CD/immunology
MH  - Cytokines/blood/immunology/metabolism
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Female
MH  - Flow Cytometry
MH  - Immunohistochemistry/veterinary
MH  - Immunophenotyping/veterinary
MH  - Interferon-beta/biosynthesis/immunology
MH  - Lymph Nodes/cytology/immunology
MH  - Lymphoid Tissue/*immunology
MH  - Male
MH  - Microscopy, Fluorescence/veterinary
MH  - Palatine Tonsil/cytology/immunology
MH  - Specific Pathogen-Free Organisms
MH  - Spleen/cytology/immunology
MH  - Swine/blood/*immunology
MH  - T-Lymphocytes/immunology
EDAT- 2006/09/19 09:00
MHDA- 2007/03/03 09:00
CRDT- 2006/09/19 09:00
PHST- 2006/03/02 00:00 [received]
PHST- 2006/06/29 00:00 [revised]
PHST- 2006/08/07 00:00 [accepted]
PHST- 2006/09/19 09:00 [pubmed]
PHST- 2007/03/03 09:00 [medline]
PHST- 2006/09/19 09:00 [entrez]
AID - S0165-2427(06)00222-4 [pii]
AID - 10.1016/j.vetimm.2006.08.009 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2006 Dec 15;114(3-4):224-37. doi: 
      10.1016/j.vetimm.2006.08.009. Epub 2006 Sep 14.