PMID- 16978777
OWN - NLM
STAT- MEDLINE
DCOM- 20061127
LR  - 20060925
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 407
IP  - 3
DP  - 2006 Oct 30
TI  - Stimulation of neurite outgrowth on neonatal cerebral astrocytes is enhanced in 
      the presence of BDNF.
PG  - 268-73
AB  - An area of increasing interest in spinal cord injury (SCI) research is the 
      development of multi-factorial strategies to promote repair. In this respect, a 
      prominent role is played by cell transplantation, the reparative effect of which 
      can be enhanced by additional use of neurotrophic factors. Immature astrocytes 
      have shown their merit in stimulating axon regeneration upon transplantation into 
      the injured spinal cord. Brain-derived neurotrophic factor (BDNF) influences a 
      wide range of descending axon tracts in the injured spinal cord. In the present 
      study, we hypothesized that the neurite outgrowth of neonatal cortical neurons on 
      immature astrocytes is enhanced in the presence of BDNF. To test this hypothesis, 
      neonatal cortical neurons were cultured on neonatal astrocytes for 2 days in 
      absence or presence of BDNF. The length of the longest neurite and the number of 
      primary neurites per neuron were taken as measures to study neurite outgrowth. We 
      show that BDNF dose-dependently enhanced neurite outgrowth of neonatal cerebral 
      cortical neurons grown on immature astrocytes. Compared to conditions without 
      BDNF, the length of the longest neurite increased by 25.5 and 28.8% in presence 
      of 10 and 100 pg/ml BDNF, respectively. BDNF did not alter the density of the 
      immature astrocytes. We conclude that the presence of BDNF enhances the neurite 
      outgrowth on immature astrocytes. A multi-factorial strategy based on 
      transplantation of neonatal astrocytes in the presence of additional BDNF is 
      recommended and may stimulate axon regrowth after experimental injury to the 
      central nervous system.
FAU - Deumens, Ronald
AU  - Deumens R
AD  - Department of Psychiatry and Neuropsychology, Division Cellular Neuroscience, 
      University of Maastricht, Maastricht, The Netherlands. r.deumens@np.unimaas.nl
FAU - Koopmans, Guido C
AU  - Koopmans GC
FAU - Jaken, Robby J P
AU  - Jaken RJ
FAU - Morren, Kathleen
AU  - Morren K
FAU - Comhair, Tine
AU  - Comhair T
FAU - Kosar, Sevginur
AU  - Kosar S
FAU - Steinbusch, Harry W M
AU  - Steinbusch HW
FAU - Den Bakker, Christel G J
AU  - Den Bakker CG
FAU - Joosten, Elbert A J
AU  - Joosten EA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060915
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Astrocytes/*drug effects/physiology
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cells, Cultured
MH  - Cerebral Cortex/*cytology
MH  - Coculture Techniques
MH  - Neurites/*drug effects/physiology
MH  - Neurons/drug effects/physiology
MH  - Rats
MH  - Rats, Inbred Lew
EDAT- 2006/09/19 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/09/19 09:00
PHST- 2006/07/03 00:00 [received]
PHST- 2006/08/19 00:00 [revised]
PHST- 2006/08/24 00:00 [accepted]
PHST- 2006/09/19 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/09/19 09:00 [entrez]
AID - S0304-3940(06)00880-9 [pii]
AID - 10.1016/j.neulet.2006.08.059 [doi]
PST - ppublish
SO  - Neurosci Lett. 2006 Oct 30;407(3):268-73. doi: 10.1016/j.neulet.2006.08.059. Epub 
      2006 Sep 15.