PMID- 16979593
OWN - NLM
STAT- MEDLINE
DCOM- 20061211
LR  - 20201209
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 349
IP  - 4
DP  - 2006 Nov 3
TI  - Regulation of IL-27p28 gene by lipopolysaccharide in dendritic DC2.4 cells.
PG  - 1372-7
AB  - To elucidate the regulation of IL-27p28 gene, we analyzed the promoter region of 
      the gene in DC2.4 cells with or without lipopolysaccharide (LPS)-treatment. The 
      results indicate that a region (-648 to -364) of p28 promoter was responsible for 
      LPS-induction. EMSA with DNA probes within the region reveals that binding of 
      GATA motif bound proteins was decreased by LPS-treatment. We identified one of 
      the proteins as non-POU domain-containing octamer binding protein (NonO). Taken 
      together, LPS-induced activation of IL-27p28 gene can be accounted for by the 
      displacement of bound NonO protein from the IL-27p28 promoter.
FAU - Kamakura, Maki
AU  - Kamakura M
AD  - Laboratory of Molecular Biology, Medical Research Center, Kochi Medical School, 
      Kochi, Japan.
FAU - Morisawa, Keiko
AU  - Morisawa K
FAU - Komi, Hideyuki
AU  - Komi H
FAU - Tomatani, Ayako
AU  - Tomatani A
FAU - Saito, Fumiji
AU  - Saito F
FAU - Konishi, Yuko
AU  - Konishi Y
FAU - Jin, Ya
AU  - Jin Y
FAU - Manabe, Takashi
AU  - Manabe T
FAU - Kuroda, Masayuki
AU  - Kuroda M
FAU - Imai, Shosuke
AU  - Imai S
FAU - Mizuguchi, Hiroyuki
AU  - Mizuguchi H
FAU - Taniguchi, Taketoshi
AU  - Taniguchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060912
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Il27 protein, rat)
RN  - 0 (Interleukins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Dendritic Cells/drug effects/*metabolism
MH  - Gene Expression Regulation/drug effects/physiology
MH  - Interleukins/genetics/*metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Mice
MH  - Recombinant Proteins/metabolism
EDAT- 2006/09/19 09:00
MHDA- 2006/12/12 09:00
CRDT- 2006/09/19 09:00
PHST- 2006/08/28 00:00 [received]
PHST- 2006/09/04 00:00 [accepted]
PHST- 2006/09/19 09:00 [pubmed]
PHST- 2006/12/12 09:00 [medline]
PHST- 2006/09/19 09:00 [entrez]
AID - S0006-291X(06)02023-7 [pii]
AID - 10.1016/j.bbrc.2006.09.004 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Nov 3;349(4):1372-7. doi: 
      10.1016/j.bbrc.2006.09.004. Epub 2006 Sep 12.