PMID- 16979595
OWN - NLM
STAT- MEDLINE
DCOM- 20061211
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 349
IP  - 4
DP  - 2006 Nov 3
TI  - ERK binds, phosphorylates InsP3 type 1 receptor and regulates intracellular 
      calcium dynamics in DT40 cells.
PG  - 1339-44
AB  - Modulation on the duration of intracellular Ca(2+) transients is essential for 
      B-cell activation. We have previously shown that extracellular-signal-regulated 
      kinase (ERK) can phosphorylate inositol 1,4,5-trisphosphate receptor type 1 
      (IP(3)R1) at serine 436 and regulate its calcium channel activity. Here we 
      investigate the potential physiological interaction between ERK and IP(3)R1 using 
      chicken DT40 B-cell line in which different mutants are expressed. The 
      interaction between ERK and IP(3)R1 is confirmed by co-immunoprecipitation and 
      fluorescence resonance energy transfer (FRET) assays. This constitutive 
      interaction is independent of either ERK kinase activation or IP(3)R1 
      phosphorylation status. Back phosphorylation analysis further shows that type 1 
      IP(3)R (IP(3)R1) is phosphorylated by ERK in anti-IgM-activated DT40 cells. 
      Finally, our data show that the phosphorylation of Ser 436 in the IP(3)-binding 
      domain of IP(3)R1 leads to less Ca(2+) release from endoplasmic reticulum (ER) 
      microsomes and accelerates the declining of calcium increase in DT40 cells in 
      response to anti-IgM stimulation.
FAU - Yang, Ling-Hai
AU  - Yang LH
AD  - Laboratory of Molecular and Developmental Biology, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, China.
FAU - Bai, Gui-Rong
AU  - Bai GR
FAU - Huang, Xiu-Ying
AU  - Huang XY
FAU - Sun, Fang-Zhen
AU  - Sun FZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060911
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Inositol 1,4,5-Trisphosphate Receptors)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
EIN - Biochem Biophys Res Commun. 2007 Mar 23;354(4):1107
MH  - Animals
MH  - B-Lymphocytes/*metabolism
MH  - Binding Sites
MH  - Calcium/*metabolism
MH  - Calcium Signaling/*physiology
MH  - Cell Line
MH  - Chickens
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Feedback/physiology
MH  - Inositol 1,4,5-Trisphosphate Receptors/*metabolism
MH  - Kinetics
MH  - Phosphorylation
MH  - Protein Binding
EDAT- 2006/09/19 09:00
MHDA- 2006/12/12 09:00
CRDT- 2006/09/19 09:00
PHST- 2006/08/29 00:00 [received]
PHST- 2006/08/30 00:00 [accepted]
PHST- 2006/09/19 09:00 [pubmed]
PHST- 2006/12/12 09:00 [medline]
PHST- 2006/09/19 09:00 [entrez]
AID - S0006-291X(06)01999-1 [pii]
AID - 10.1016/j.bbrc.2006.08.185 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Nov 3;349(4):1339-44. doi: 
      10.1016/j.bbrc.2006.08.185. Epub 2006 Sep 11.