PMID- 16979873
OWN - NLM
STAT- MEDLINE
DCOM- 20070227
LR  - 20101001
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 19
IP  - 2
DP  - 2007 Feb
TI  - Upregulated expression of kappa light chain by Epstein-Barr virus encoded latent 
      membrane protein 1 in nasopharyngeal carcinoma cells via NF-kappaB and AP-1 
      pathways.
PG  - 419-27
AB  - B lymphocytes are generally considered to be the only source of immunoglobulins. 
      However, increasing evidence revealed that some human epithelial cancer cell 
      lines, including nasopharyngeal carcinoma (NPC) cell lines, expressed 
      immunoglobulins. Moreover, we previously found that expression of kappa light 
      chain in NPC cells could be upregulated by EBV-encoded latent membrane protein 1 
      (LMP1). Here, Western blot and flow cytometric analysis of intracellular kappa 
      staining indicated that upregulation of the expression of kappa was inhibited by 
      using LMP1-targeted DNAzyme and that Bay11-7082 and SP600125, inhibitors of JNK 
      and NF-kappaB, respectively, inhibited LMP1-augmented kappa light chain 
      expression in NPC cells. LMP1-positive NPC cells expressing the dominant-negative 
      mutant of IkappaBalpha (DNMIkappaBalpha) or of c-Jun (TAM67) exhibited 
      significantly decreasing kappa production compared with their parental cells. 
      These results suggest that LMP1 elevated kappa light chain through activation of 
      the NF-kappaB and AP-1 signaling pathways. The present study provided some hints 
      of possible mechanisms by which human cancer cells of epithelial origin produced 
      immunoglobulins.
FAU - Liu, Hai-dan
AU  - Liu HD
AD  - Cancer Research Institute, Xiangya School of Medicine, Central South University, 
      Xiangya Road #110, Changsha, Hunan 410078, PR China.
FAU - Zheng, Hui
AU  - Zheng H
FAU - Li, Ming
AU  - Li M
FAU - Hu, Duo-sha
AU  - Hu DS
FAU - Tang, Min
AU  - Tang M
FAU - Cao, Ya
AU  - Cao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060918
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (EBV-associated membrane antigen, Epstein-Barr virus)
RN  - 0 (I kappa B beta protein)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Peptide Fragments)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (RNA, Messenger)
RN  - 0 (TAM67 peptide)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Cell Line, Tumor
MH  - Humans
MH  - I-kappa B Proteins/genetics
MH  - NF-kappa B/*metabolism
MH  - Nasopharyngeal Neoplasms/*metabolism
MH  - Peptide Fragments/metabolism
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - RNA, Messenger/metabolism
MH  - Signal Transduction
MH  - Transcription Factor AP-1/*metabolism
MH  - Transfection
MH  - *Up-Regulation
MH  - Viral Matrix Proteins/chemistry/*metabolism
EDAT- 2006/09/19 09:00
MHDA- 2007/02/28 09:00
CRDT- 2006/09/19 09:00
PHST- 2006/07/13 00:00 [received]
PHST- 2006/07/24 00:00 [accepted]
PHST- 2006/09/19 09:00 [pubmed]
PHST- 2007/02/28 09:00 [medline]
PHST- 2006/09/19 09:00 [entrez]
AID - S0898-6568(06)00174-4 [pii]
AID - 10.1016/j.cellsig.2006.07.012 [doi]
PST - ppublish
SO  - Cell Signal. 2007 Feb;19(2):419-27. doi: 10.1016/j.cellsig.2006.07.012. Epub 2006 
      Sep 18.