PMID- 16980038
OWN - NLM
STAT- MEDLINE
DCOM- 20061208
LR  - 20191224
IS  - 0041-1345 (Print)
IS  - 0041-1345 (Linking)
VI  - 38
IP  - 7
DP  - 2006 Sep
TI  - The C-terminal peptide of connective tissue growth factor blocks the full 
      molecule binding to tubular epithelial cell.
PG  - 2187-9
AB  - OBJECTIVES: Interstitial fibrosis is a critical pathologic change in chronic 
      allograft nephropathy. The cytokine connective tissue growth factor (CTGF, also 
      CCN2) plays an important role in epithelial-mesenchymal transformation (EMT) of 
      tubular epithelial cells to renal interstitial fibrosis. The hexadeca-peptide 
      within the C-terminal of CTGF (named P2) contains the unique binding domain of 
      CTGF to its potential receptor, integrin alphavbeta3. This study examined whether 
      P2 bound preferentially to the receptor and served as an inhibitor of CTGF. 
      METHODS: All studies used an established rat kidney tubular epithelial cell line 
      NRK-52E. Chemically synthesized P2 was purified, and some of it labeled with 
      FITC. The affinity of CTGF or P2 to NRK-52E cells was examined by a solid-phase 
      cell adhesion assay. Competitive binding between P2 and CTGF to NRK-52E cells was 
      examined with flow cytometric analysis. RESULTS: Both P2 and CTGF bound to the 
      NRK-52E cells, mediating cell adhesion. When the cells were incubated in the 
      mixture of P2 and CTGF, P2 bound to the cells preferentially. Furthermore, when 
      cells were preincubated with excessive CTGF, it became difficult for subsequent 
      P2 binding to occur. CONCLUSIONS: P2 and CTGF seemed to bind to cell membranes at 
      the same binding domain. P2 competitively blocked CTGF binding, acting as a CTGF 
      inhibitor.
FAU - Shi, Y
AU  - Shi Y
AD  - Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West 
      China Hospital, SiChuan University, Guoxuexiang 37, Chengdu 610041, P.R. China.
FAU - Wang, W
AU  - Wang W
FAU - Tu, Z
AU  - Tu Z
FAU - Zhang, L
AU  - Zhang L
FAU - Qiu, J
AU  - Qiu J
FAU - Li, Q
AU  - Li Q
FAU - Ye, F
AU  - Ye F
FAU - Li, S
AU  - Li S
FAU - Bu, H
AU  - Bu H
FAU - Li, Y
AU  - Li Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (CCN2 protein, human)
RN  - 0 (CCN2 protein, rat)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Peptide Fragments)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Binding, Competitive
MH  - Cell Adhesion/drug effects/physiology
MH  - Cell Line
MH  - Connective Tissue Growth Factor
MH  - Humans
MH  - Immediate-Early Proteins/chemistry/*pharmacology
MH  - Intercellular Signaling Peptides and Proteins/chemistry/*pharmacology
MH  - Kidney Tubules/drug effects/*physiology
MH  - Kinetics
MH  - Peptide Fragments/*pharmacology
MH  - Rats
MH  - Urothelium/drug effects/*physiology
EDAT- 2006/09/19 09:00
MHDA- 2006/12/12 09:00
CRDT- 2006/09/19 09:00
PHST- 2006/09/19 09:00 [pubmed]
PHST- 2006/12/12 09:00 [medline]
PHST- 2006/09/19 09:00 [entrez]
AID - S0041-1345(06)00739-1 [pii]
AID - 10.1016/j.transproceed.2006.06.093 [doi]
PST - ppublish
SO  - Transplant Proc. 2006 Sep;38(7):2187-9. doi: 10.1016/j.transproceed.2006.06.093.