PMID- 16980714
OWN - NLM
STAT- MEDLINE
DCOM- 20061219
LR  - 20220223
IS  - 1340-2838 (Print)
IS  - 1340-2838 (Linking)
VI  - 13
IP  - 3
DP  - 2006 Jun 30
TI  - Genomic instability of the DYZ1 repeat in patients with Y chromosome anomalies 
      and males exposed to natural background radiation.
PG  - 103-9
AB  - We assessed genomic instability of 3.4 kb DYZ1 repeat arrays in patients 
      encompassing prostate cancer (PC), cases of repeated abortion (RA) and males 
      exposed to natural background radiation (NBR) using real-time PCR and 
      fluorescence in situ hybridization (FISH). Normal males showed DYZ1 copies 
      ranging from 3000 to 4300, RA, 0-2237; PC, 550; and males exposed to NBR, 
      1577-5700. FISH showed organizational variation of DYZ1 in these samples 
      substantiating the data obtained from real-time PCR. Of the 10 RA samples, 7 were 
      found to be affected of which, 5 showed deletion of 265 bp from nt 25 to 290 and 
      773 bp from 1347 to 2119 and 2 showed deletion of 275 bp from nt 3128 to 3402. 
      Copy number variation of DYZ1 in these males correlated with genetic 
      constrains/anomalies. Although precise mechanisms of genomic instability of DYZ1 
      remains unclear, we construe that this repeat plays a critical role in 
      maintaining the structural integrity of the Y chromosome, possibly by absorbing 
      the load of mutations. This may be used as a marker system to analyze genetic 
      integrity of the DYZ1 repeat array(s) across the spectrum of patients.
FAU - Pathak, Deepali
AU  - Pathak D
AD  - Molecular Genetics Laboratory, National Institute of Immunology Aruna Asaf Ali 
      Marg, New Delhi 11 0067, India.
FAU - Premi, Sanjay
AU  - Premi S
FAU - Srivastava, Jyoti
AU  - Srivastava J
FAU - Chandy, Sebastian Padinjarel
AU  - Chandy SP
FAU - Ali, Sher
AU  - Ali S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060916
PL  - England
TA  - DNA Res
JT  - DNA research : an international journal for rapid publication of reports on genes 
      and genomes
JID - 9423827
SB  - IM
MH  - Abortion, Induced
MH  - Background Radiation
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Y
MH  - Female
MH  - Gene Dosage
MH  - *Genomic Instability
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Prostatic Neoplasms/diagnosis/genetics
MH  - *Repetitive Sequences, Nucleic Acid
EDAT- 2006/09/19 09:00
MHDA- 2006/12/21 09:00
CRDT- 2006/09/19 09:00
PHST- 2006/09/19 09:00 [pubmed]
PHST- 2006/12/21 09:00 [medline]
PHST- 2006/09/19 09:00 [entrez]
AID - dsl002 [pii]
AID - 10.1093/dnares/dsl002 [doi]
PST - ppublish
SO  - DNA Res. 2006 Jun 30;13(3):103-9. doi: 10.1093/dnares/dsl002. Epub 2006 Sep 16.